• Species Reactivity

  Mouse

• Specificity

  Detects mouse Granzyme G in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross‑reactivity with recombinant mouse Granzyme D is observed and less than 5% cross-reactivity with recombinant human Granzyme H and recombinant mouse Granzyme B is observed.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  S. frugiperda insect ovarian cell line     Sf 21-derived recombinant mouse Granzyme G    
  Ile21-Leu248    
  Accession # P13366

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  0.1 µg/mL

  Recombinant Mouse Granzyme G (Catalog # )

• 
  

• Immunoprecipitation

  25 µg/mL

  Conditioned cell culture medium spiked with Recombinant Mouse Granzyme G (Catalog # ),

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Granzyme G

Granzyme G is a member of the Granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells (1). Together with Granzymes D, E and F, it is regulated through pregnancy and by IL-2 and IL-15 in granulated metrial gland cells (2). Human or rat counterpart of mouse Granzyme G has not been found. Like other Granzymes, mouse Granzyme G is not secreted as a zymogen but stored as a fully processed and activated enzyme in the cytoplasmic granules of CTL (3). It is synthesized as a 248 amino acid precursor with a 18 amino acid signal peptide and a 2 amino acid propeptide (3, 4). The mature protein (residues 21‑248) is expressed and purified.

• References:

1. Smyth, M.J. et al. (1996) J. Leukoc. Biol. 60:555.

2. Allen, M.P. and M. Nilsen-Hamilton (1998) J. Immunol. 161:2772.

3. Jenne, D.E. et al. (1989) Biochemistry 28:7953.

4. Kwon, B.S. et al. (1988) J. Exp. Med. 168:1839.

• Entrez Gene IDs:

  14944 (Mouse); 266704 (Rat)

• Alternate Names:

  Ctla7; Ctla-7; Granzyme G; GrzG; GZMG