• Species Reactivity

  Mouse

• Specificity

  Detects mouse IGSF4A/SynCAM1 in direct ELISAs and Western blots.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse IGSF4A/SynCAM1    
  Ile42-Asp376    
  Accession # NP_061240

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  0.1 µg/mL

  Recombinant Mouse IGSF4A/SynCAM1 (Catalog # )

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGSF4A/SynCAM1

IGSF4A is a 75 kDa immunoglobulin superfamily protein that mediates calcium-independent adhesive interactions, either homophilic or heterophilic with CRTAM (class I-restricted T cell-associated molecule), Necl-1 (SynCAM3) or Nectin-3 (1‑4). Alternate names for IGSF4A reflect its activities. As TSLC-1 (tumor suppressor in lung cancer-1), it is downregulated in non-small cell lung cancer (5). This may allow evasion of antitumor responses, since TSLC-1 interaction with CRTAM on activated NK and CD8⁺ T cells stimulates cytotoxicity and upregulates IFN-gamma production (3, 6). As Necl-2 (nectin-like 2), it is expressed on T zone-restricted BDCA3⁺ dendritic cells, again interacting with CRTAM (3). As SynCAM-1 (synaptic cell adhesion molecule-1) it binds homotypically across synaptic clefts and, like neuroligin/neurexin adhesion pairs, promotes formation of neural cell synapses (7, 8). Expression on mast cells allows attachment to neurites and fibroblasts (9, 10). As SgIGSF (spermatogenic IgSF), expression on developing sperm allows adhesion to an unknown partner on Sertoli cells that is critical for normal development (11, 12). Mouse IGSF4A cDNA encodes 445 amino acids including a signal peptide, three Ig-like domains (a V-type, a divergent C1-type and an I-type), a transmembrane domain and a cytoplasmic domain with protein 4.1 and PDZ domain binding sites. These sites allow connections to adaptor molecules and are critical in synaptogenesis (7, 8). The outer Ig-like domain most likely mediates adhesion, with altered affinity for splice variants that have either shorter spacing of this domain from the plasma membrane or varied glycosylation (1, 6). The six reported splice variants include a prominently expressed soluble form of about 45 kDa that inhibits adhesion (13). Mouse IGSF4A ECD shares 99%, 96%, 98% and 88% amino acid identity with rat, human, canine, and bovine IGSF4A, respectively.

• References:

1. Biederer, T. et al. (2006) Genomics 87:139.

2. Wakayama, T. et al. (2001) Mol. Reprod. Dev. 60:158.

3. Galibert, L. et al. (2005) J. Biol. Chem. 280:21955.

4. Shingai, T. et al. (2003) J. Biol. Chem. 278:35421.

5. Kuramochi, M. et al. (2002) Nat. Genet. 27:427.

6. Boles, K.S. et al. (2005) Blood 106:779.

7. Biederer, T. et al. (2002) Science 297:1525.

8. Sara, Y. et al. (2005) J. Neurosci. 25:260.

9. Furuno, T. et al. (2005) J. Immunol. 174:6934.

10. Ito, A. et al. (2003) Blood 101:2601.

11. Wakayama, T. et al. (2001) Mol. Reprod. Dev. 60:158.

12. Fujita, E. et al. (2006) Mol. Cell. Biol. 26:718.

13. Koma, Y. et al. (2004) Oncogene 23:5687.

• Long Name:

  Immunoglobulin Superfamily, Member 4

• Entrez Gene IDs:

  23705 (Human); 16151 (Mouse)

• Alternate Names:

  BL2; Cadm1; cell adhesion molecule 1; IgSF4; IGSF4A; IGSF4ADKFZp686F1789; IGSF4immunoglobulin superfamily, member 4D variant 2; Immunoglobulin superfamily member 4; immunoglobulin superfamily, member 4; Necl-2; NECL2MGC149785; nectin-like 2; Nectin-like protein 2; RA175; sgIGSF; Spermatogenic immunoglobulin superfamily; ST17; sTSLC-1; Synaptic cell adhesion molecule; SynCAM; SynCAM1; SYNCAMMGC51880; TSLC1; TSLC-1; TSLC1/Nectin-like 2/IGSF4; Tumor suppressor in lung cancer 1SYNCAM1