• Species Reactivity

  Mouse

• Specificity

  Detects mouse Cryptic in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human Cryptic is observed.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse Cryptic    
  Tyr36-Gly177    
  Accession # P97766

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  0.1 µg/mL

  Recombinant Mouse Cryptic

• 
  

• Flow Cytometry

  2.5 µg/10    ⁶ cells

  D3 mouse embryonic stem cell line

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cryptic

Cryptic, also known as CriptoFRL1Cryptic (CFC-1), was originally identified as a secreted protein that is specifically expressed during mesoderm differentiation. Cryptic, along with Cripto, Xenopus FRL-1 and zebrafish OEP (one-eyed pinhead) define the epidermal growth factor-CriptoFRL1Cryptic (EGF-CFC) family of signaling proteins. Genetic evidence from mice and humans points to a role for cryptic in determining left-right asymmetry.

• Entrez Gene IDs:

  55997 (Human); 12627 (Mouse)

• Alternate Names:

  CFC1; CFC-1; CFC1B; cripto, FRL-1, cryptic family 1; Cryptic family protein 1; cryptic protein; Cryptic; CRYPTICFLJ77897; HTX2MGC133213