• Species Reactivity

  Mouse

• Specificity

  Detects mouse TIM-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross‑reactivity with recombinant human TIM-1 and recombinant mouse TIM-3 is observed.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse TIM‑2    
  His22-Asn229    
  Accession # NP_599010

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  0.1 µg/mL

  Recombinant Mouse TIM‑2

• 
  

• Immunohistochemistry

  1-15 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TIM-2

TIM-2 is a type I membrane protein and is a member of the TIM protein family. This protein family is characterized by their conserved immunoglobulin and mucin domains, as well as by their expression on activated T cells. TIM-2 is a receptor for Sema 4A, which is expressed on dendritic cells and B cells. Sema 4A has been shown to enhance T cell activation.

• Long Name:

  T Cell Immunoglobulin Mucin-2

• Entrez Gene IDs:

  171284 (Mouse)

• Alternate Names:

  T Cell immunoglobulin and mucin domain protein 2; TIM2; TIM-2