• Species Reactivity

  Mouse

• Specificity

  Detects mouse JMJD6/PSR in direct ELISAs and Western blots.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse JMJD6/PSR    
  Ala289-Arg403    
  Accession # Q9ERI5

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  0.1 µg/mL

  Recombinant Mouse JMJD6/PSR

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: JMJD6/PSR

JMJD6 (Jumonji [Japanese for "cruciform/cross"] domain-containing protein 6; also PTDSR/PSR and Lysyl-hydroxylase JMJD6) is a 47-55 kDa member of the JMJD6 family, cupin superfamily of proteins. It is induced by hypoxia, and expressed in skeletal muscle, macrophages and possibly endothelial cells. JMJD6 is reported to demethylate histones (which it does not), serve as a phosphatidyl serine receptor (which it might), and act as a lysyl hydroxylase (which it does). In the nucleus, it appears to regulate pre-RNA splicing by hydroxylating various splicing factors such as U2AF65. Mouse JMJD6 is 403 amino acids (aa) in length. It contains five NLS's plus a JmjC domain (aa 141-305) and three iron-based catalytic sites. There at least three potential isoform variants. One contains a 29 aa substitution for aa 270‑403, a second possesses a 30 aa substitution for aa 302-314, and a third shows a 21 aa substitution for aa 315-403. JMJD6 is known to form high MW homo‑trimers, -tetramers and -oligomers. Full-length mouse JMJD6 shares 98% and 99% aa sequence identity with human and rat JMJD6, respectively.

• Long Name:

  Jumonji Domain Containing 6/Phosphatidylserine Receptor

• Entrez Gene IDs:

  23210 (Human); 107817 (Mouse); 360665 (Rat)

• Alternate Names:

  bifunctional arginine demethylase and lysyl-hydroxylase JMJD6; EC 1.14.11; EC 1.14.11.-; Histone arginine demethylase JMJD6; JmjC domain-containing protein 6; JMJD6; jumonji domain containing 6; Jumonji domain-containing protein 6; KIAA0585PSR; Lysyl-hydroxylase JMJD6; Peptide-lysine 5-dioxygenase JMJD6; Phosphatidylserine receptorPTDSR; Protein PTDSR; PSR; PTDSR; PTDSR1