• Species Reactivity

  Mouse

• Specificity

  Detects mouse IL-12 R beta 1 in direct ELISAs and Western blots. In these formats, approximately 20% cross‑reactivity with recombinant human IL-12 R beta 1 is observed.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  S. frugiperda insect ovarian cell line     Sf 21-derived recombinant mouse IL-12 R beta 1    
  Val32-Glu561    
  Accession # Q60837

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Endotoxin Level

  <0.10 EU per 1 μg of the antibody by the LAL method.  

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  0.1 µg/mL

  Recombinant Mouse IL‑12 R beta 1 (Catalog # )

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-12 R beta 1

IL-12 R beta 1 is a 100 kDa type I transmembrane protein that belongs to the gp130/G-CSF R family of cytokine receptors. IL-12 R beta 1 is a common subunit of both the IL‑12 and IL-23 receptor complexes which play distinct but related roles in T cell mediated inflammatory reactions (1, 2). Mature mouse IL-12 R beta 1 contains a 546 amino acid (aa) extracellular domain (ECD) with five fibronectin type III repeats, and a 147 aa cytoplasmic domain (3). Within the ECD, mouse IL-12 R beta 1 shares 85% and 52% aa sequence identity with rat and human IL-12 R beta 1, respectively. It shares 16% - 21% aa sequence identity with the ECDs of mouse gp130, LIF R, G-CSF R, and IL-23 R. IL-12 and IL-23 are disulfide linked heterodimeric cytokines that share a common p40 subunit (1, 2). IL-12 R beta 1 interacts with p40 at low affinity but does not transmit signals (3). Increased ligand binding affinity and signaling capacity are gained by association of IL-12 R beta 1 with either IL-12 R beta 2 or IL-23 R (4 - 6). IL-12 R beta 2 and IL-23 R are the signal transducing components of these receptor complexes (4, 7). IL-12 R beta 1 is expressed on activated T cells, NK cells, B cells, macrophages, and microglia (8 - 10). IL-12 induced signaling promotes the development of naïve T cells into IFN-beta producing Th1 cells (11). IL-23 contributes to chronic inflammation by inducing the production of IL-17 by memory T cells (12). Naturally occurring homodimers of p40 can function as antagonists of IL-12 and IL‑23 and can also induce macrophage chemotaxis in the absence of IL-12 R beta 2 (13, 14).

• References:

1. Becker, C. et al. (2005) Inflamm. Bowel Dis. 11:755.

2. Hunter, C.A. (2005) Nat. Rev. Immunol. 5:521.

3. Chua, A.O. et al. (1995) J. Immunol. 155:4286.

4. Parham, C. et al. (2002) J. Immunol. 168:5699.

5. Wu, C. et al. (1997) J. Immunol. 159:1658.

6. Zou, J. et al. (1997) J. Biol. Chem. 272:6073.

7. Presky, D.H. et al. (1996) Proc. Natl. Acad. Sci. 93:14002.

8. Wu, C. et al. (1997) Eur. J. Immunol. 27:147.

9. Airoldi, I. et al. (2000) J. Immunol. 165:6880.

10. Li, J. et al. (2003) J. Neurol. Sci. 215:95.

11. Schmitt, E. et al. (1994) Eur. J. Immunol. 24:793.

12. Yen, D. et al. (2006) J. Clin. Invest. 116:1310.

13. Shimozato, O. et al. (2006) Immunology 117:22.

14. Russell, T.D. et al. (2003) J. Immunol. 171:6866.

• Long Name:

  Interleukin 12 Receptor beta 1

• Entrez Gene IDs:

  3594 (Human); 16161 (Mouse)

• Alternate Names:

  CD212 antigen; CD212; IL-12 R beta 1; IL-12 receptor beta component; IL-12 receptor subunit beta-1; IL12R beta 1; IL-12R subunit beta-1; IL12R; IL12RB; IL12RB1; IL-12Rb1; IL-12R-BETA1; IL-12R-beta-1; interleukin 12 receptor, beta 1; interleukin-12 receptor beta-1 chain; interleukin-12 receptor subunit beta-1; MGC34454