• Species Reactivity

  Mouse

• Specificity

  Detects mouse Wnt-10b in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant mouse (rm) Wnt-3a, rmWnt-4, rmWnt-5a, and rmWnt-8a is observed.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  E. coli-derived recombinant mouse Wnt-10b peptide    
  Leu42-Glu82, Arg263-Arg332    
  Accession # P48614

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  0.1 µg/mL

  Recombinant Mouse Wnt‑10b

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Wnt-10b

Wnt-10b (also known as Wnt-12) is a 45 kDa glycoprotein involved in fetal limb bud development and adult hematopoiesis. Mouse Wnt-10b exists as two isoforms: a 361 amino acid (aa) long form and a 265 aa short form that lacks a 95 aa internal sequence. Wnt-10b is less than 60% aa identical to other Wnts, but mouse to human, Wnt-10b shows almost complete aa sequence identity at > 97%.

• Long Name:

  Wingless-type MMTV Integration Site Family, Member 10B

• Entrez Gene IDs:

  7480 (Human); 22410 (Mouse)

• Alternate Names:

  protein Wnt-10b; Protein Wnt-12; SHFM6WNT-10B protein; wingless-type MMTV integration site family, member 10B; Wnt10b; Wnt-10b; WNT12; Wnt-12