• Species Reactivity

  Mouse

• Specificity

  Detects mouse ADAM33 Ectodomain in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) ADAM8, rmADAM10, and rmADAM15 is observed.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse ADAM33 Ectodomain

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  0.1 µg/mL

  Recombinant Mouse ADAM33 Ectodomain

• 
  

• Immunoprecipitation

  25 µg/mL

  Conditioned cell culture medium spiked with Recombinant Mouse ADAM33,

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ADAM33

Members of the ADAM or MDC (Metalloprotease, Disintegrin, Cysteine-rich) family contain pro, metalloprotease-like, disintegrin-like, cysteine-rich, transmembrane and cytoplasmic domains. They play a fundamental role in diverse processes such as asthma, development, angiogenesis and cancer through their activities in cell adhesion/fusion, membrane protein shedding, and signal transduction. Over 30 members have been identified and about half of them are active metalloproteases such as ADAM8, 9, 10, 12 and 17/TACE.

• Long Name:

  A Disintegrin and Metalloproteinase Domain 33

• Entrez Gene IDs:

  80332 (Human); 110751 (Mouse)

• Alternate Names:

  a disintegrin and metalloprotease 33; a disintegrin and metalloproteinase domain 33; ADAM 33; ADAM metallopeptidase domain 33; ADAM33; C20orf153; chromosome 20 open reading frame 153; disintegrin and metalloproteinase domain-containing protein 33; disintegrin and reprolysin metalloproteinase family protein; dJ964F7.1; DKFZp434K0521; EC 3.4.24.-; FLJ35308; FLJ36751; MGC149823; MGC71889