• Species Reactivity

  Mouse

• Specificity

  Detects mouse Netrin-G2a in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant mouse (rm) Netrin-1, rmNetrin-G1a, and rmNetrin-4 is observed and less than 1% cross-reactivity with recombinant chicken Netrin-2 is observed.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse Netrin-G2a    
  Asp18-Arg504    
  Accession # NP_598007

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  0.1 µg/mL

  Recombinant Mouse Netrin-G2a

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Netrin-G2a

Mouse Netrin-G2a is a member of a GPI-linked Netrin subfamily that is distantly related to classical Netrins. Netrin-G2a is approximately 485 amino acids (aa) in length. It contains an N-terminal laminin-related region plus a heparin-binding C-terminus that ends in a GPI-linkage. The laminin-related region shows a globular type VI domain followed by three variable-length EGF‑like domains. Alternate splicing generates two larger isoforms. Amino acid insertions of 59 aa and 34 aa between EGF‑like motifs 1 and 2 generate Netrin-G2b and Netrin-G2c,respectively. Mature mouse Netrin-G2a shares 96% and 99% aa identity with human Netrin-G2 and the equivalent region in rat Netrin-G2b, respectively.

• Entrez Gene IDs:

  84628 (Human); 171171 (Mouse)

• Alternate Names:

  Lmnt2; NetrinG2a; Netrin-G2a; NTG2; NTNG2