• Species Reactivity

  Mouse

• Specificity

  Detects mouse Aminopeptidase A/ENPEP in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human ENPEP is observed.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse Aminopeptidase A/ENPEP    
  Arg41-Pro945    
  Accession # P16406

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  0.1 µg/mL

  Recombinant Mouse Aminopeptidase A/ENPEP

• 
  

• Immunoprecipitation

  25 µg/mL

  Conditioned cell culture medium spiked with Recombinant Mouse Aminopeptidase A/ENPEP,

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Aminopeptidase A/ENPEP

The mouse ENPEP gene encodes Aminopeptidase A (APA), which is also known as glutamyl aminopeptidase (EAP), aspartate aminopeptidase, angiotensinase A, Ca²⁺-activated glutamate aminopeptidase, membrane aminopeptidase II and the BP-1/6C3 antigen (2). The various names reflect its substrate specificity (prefer Glu or Asp and angiotensin II as one of the physiological substrates), Ca²⁺-dependence, cellular location (type II membrane protein) and immunological reactivity to antibody BP-1/6C3. The deduced amino acid sequence of mouse ENPEP consists of a short cytoplasmic tail (residues 1 to 18), a transmembrane region (residue 19 to 39), and a long ectodomain (residues 40 to 945) (1). In addition to the N-terminal zinc metalloprotease domain, the ectodomain also contains the C‑terminal region, which functions as an intramolecular chaperone required for the correct folding, cell surface expression and activity (3). The purified rmENPEP consists of the ectodomain and is active in the assay described above.

• References:

1. Wu, Q. et al. (1990) Proc. Natl. Acad. Sci. USA 87:993.

2. Wang, J. and M.D. Cooper (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) pp. 299, Academic Press, San Diego.

3. Rozenfeld, R. et al. (2004) J. Biol. Chem. 279:43285.

• Entrez Gene IDs:

  2028 (Human); 13809 (Mouse)

• Alternate Names:

  Aminopeptidase A; APA; AP-A; CD249 antigen; CD249; Differentiation antigen gp160; EAP; EC 3.4.11; EC 3.4.11.7; ENPEP; glutamyl aminopeptidase (aminopeptidase A); glutamyl aminopeptidase; gp160