• Species Reactivity

  Mouse

• Specificity

  Detects mouse BACE-2 Ectodomain in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 15% cross‑reactivity with recombinant mouse BACE and recombinant human BACE-2 is observed.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse BACE-2    
  Phe29-Pro462 (Tyr357His)    
  Accession # Q9JL18

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  0.1 µg/mL

  Recombinant Mouse BACE‑2 (Catalog # )

• 
  

• Immunoprecipitation

  25 µg/mL

  Conditioned cell culture medium spiked with Recombinant Mouse BACE‑2 (Catalog # ),

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BACE-2

BACE-2 is an aspartic protease that shares sequence similarity with BACE-1, the major beta -secretase for generation of A beta peptide in neurons (1). However, BACE-2 differs from BACE-1 in several aspects including pro-enzyme activation, substrate binding sites, transcriptional regulation, and function in A beta peptide production (2‑4). The domain structure of mouse BACE-2 consists of a signal peptide (aa 1‑19), pro (aa 20‑62) and a mature chain (aa 63‑514) that consists of a transmembrane domain and a cytoplasmic tail. The C-terminal region (aa 463‑514) was replaced by a His tag in the recombinant mouse BACE-2 that also contained a His residue at the position of Tyr357. The purified recombinant mouse BACE-2 started at Ala63, the same residue shown at the N-terminus of the human active BACE-2 (2).

• References:

1. Cai, H. et al. (2001) Nature Neurosci. 4:233.

2. Hussain, I. et al. (2001) J. Biol. Chem. 276:23322.

3. Ostermann, N. et al. (2006) J. Mol. Biol. 355:249.

4. Sun, X. et al. (2005) FASEB J. 19:739.

• Long Name:

  beta-site Ayloid Precursor Protein Cleaving Enzyme 2

• Entrez Gene IDs:

  25825 (Human); 56175 (Mouse); 288227 (Rat)

• Alternate Names:

  AEPLC; Asp 1; ASP1; ASP21; Aspartic-like protease 56 kDa; Aspartyl protease 1; BACE2; BACE-2; beta secretase 2; beta-secretase 2; beta-site amyloid beta A4 precursor protein-cleaving enzyme 2; Beta-site amyloid precursor protein cleaving enzyme 2; Beta-site APP cleaving enzyme 2; beta-site APP-cleaving enzyme 2; beta-site APP-cleaving enzyme 2, EC 3.4.2310ALP5656 kDa aspartic-like protease; CEAP1; Down region aspartic protease; Down syndrome region aspartic protease; DRAPBAE2; EC 3.4.23; EC 3.4.23.45; memapsin-1; Membrane-associated aspartic protease 1; theta-secretase; transmembrane aspartic proteinase Asp1