• Species Reactivity

  Mouse

• Specificity

  Detects mouse APLP-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 45% cross-reactivity with recombinant human APLP-1 is observed and less than 1% cross-reactivity with recombinant mouse APLP-2 is observed.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse APLP-1    
  Gly37-Arg582    
  Accession # Q03157

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  0.1 µg/mL

  Recombinant Mouse APLP‑1

• 
  

• Immunoprecipitation

  25 µg/mL

  Conditioned cell culture medium spiked with Recombinant Mouse APLP‑1,

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: APLP-1

APLP-1 is a transmembrane metalloprotein that is expressed in central neurons. Similar to APP and APLP-2, APLP-1 is susceptible to cleavage by various secretases, generating multiple fragments from the extracellular and intracellular domains. These include peptides similar to the amyloidogenic A beta peptides and a cytoplasmic fragment that associates with Fe65 family proteins and functions as a transcriptional activator. The extracellular domain contains heparin and collagen binding regions and is 89% identical between human and mouse.

• Long Name:

  Amyloid-like Protein 1

• Entrez Gene IDs:

  1803 (Human); 333 (Human); 502317 (Rat)

• Alternate Names:

  amyloid beta (A4) precursor-like protein 1; amyloid precursor-like protein 1; amyloid-like protein 1; APLP1; APLP-1; APLPAPLP-1