• Species Reactivity

  Mouse

• Specificity

  Detects mouse Matrilin-2 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross‑reactivity with recombinant human Matrilin-2 is observed and less than 5% cross-reactivity with recombinant mouse  (rm) Matrilin-3 and rmMatrilin-4 is observed.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse Matrilin-2    
  Arg24-Arg956    
  Accession # AAH05429

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  0.1 µg/mL

  Recombinant Mouse Matrilin‑2 (Catalog # )

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Matrilin-2

Matrilin-2 is an extracellular matrix protein that belongs to the superfamily of von Willebrand factor A domain (VWA) containing proteins. It is expressed in many tissues and functions as a bridging component between other matrix molecules (1, 2, 3, 4). The mouse Matrilin‑2 cDNA encodes a 956 amino acid (aa) precursor with a 23 aa signal sequence, two VWA domains separated by ten tandem EGF-like repeats, and a C‑terminal coiled coil domain (5). Mouse Matrilin-2 shares 84%‑87% aa sequence identity with human, rat, and canine Matrilin-2, and 26%, 21%, and 34% aa sequence identity with mouse Matrilin-1, -3, and -4, respectively. Matrilin-2 forms a variety of disulfide-linked oligomers via its coiled coil domain (4, 6‑8). It can assemble into homotrimers or heterotrimers with Matrilin-1 and/or Matrilin-4 (4, 6, 7) but has not been detected in heterotrimers containing Matrilin-3 (7). The VWA domains are thought to mediate Matrilin-Matrilin interactions as well as interactions with other matrix proteins such as Fibronectin, Collagen I, Fibrilin-2, and Laminin-1/Nidogen-1 complexes (6). Matrilin-2 knockout mice do not display any obvious abnormalities, suggesting that the expression of other molecules can compensate for the lack of Matrilin-2 (9).

• References:

1. Wagener, R. et al. (2005) FEBS Lett. 579:3323.

2. Deak, F. et al. (1999) Matrix Biol. 18:55.

3. Whittaker, C.A. and R.O. Hynes (2002) Mol. Biol. Cell 13:3369.

4. Piecha, D. et al. (1999) J. Biol. Chem. 274:13353.

5. Deak, F. et al. (1997) J. Biol. Chem. 272:9268.

6. Piecha, D. et al. (2002) Biochem. J. 367:715.

7. Frank, S. et al. (2002) J. Biol. Chem. 277:19071.

8. Pan, O.H. and K. Beck (1998) J. Biol. Chem. 273:14205.

9. Mates, L. et al. (2004) Matrix Biol. 23:195.

• Entrez Gene IDs:

  4147 (Human)

• Alternate Names:

  MATN2; matrilin 2; Matrilin2; Matrilin-2