• Species Reactivity

  Mouse

• Specificity

  Detects mouse DPPII/QPP/DPP7 in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human DPPII is observed.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse DPPII/QPP/DPP7    
  Asp34-Arg506    
  Accession # NP_114031

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  0.1 µg/mL

  Recombinant Mouse DPPII/QPP/DPP7 (Catalog # )

• 
  

• Immunoprecipitation

  25 µg/mL

  Conditioned cell culture medium spiked with Recombinant Mouse DPPII/QPP/DPP7 (Catalog # ),

• 
  

• Neutralization

  Measured by its ability to neutralize Recombinant Mouse DPPII/QPP/DPP7 (0.02 µg/mL, Catalog # ) cleavage of the fluorogenic peptide substrate KP-Amc (10 µM). The Neutralization Dose (ND    ₅₀) is typically 0.45 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DPPII/QPP/DPP7

Dipeptidyl-peptidase II (DPPII) is identical to quiescent cell proline dipeptidase (QPP) and dipeptidylpeptidase 7 (DPP7) (1, 2). It shares some substrate and cleavage specificity with DPPIV/CD26, DPP8, DPP9 and seprase/FAP (fibroblast activation protein), members of the S09 family of serine proteases. As prolyl proteases that cleave proteins and peptides after proline residues, these enzymes have a high potential for drug discovery (3, 4). However, DPP7 is not a member of the S09 family, but a member of the S28 family that also includes lysosomal Pro-X carboxypeptidase/prolylcarboxypeptidase/PRCP and thymus-specific serine peptidase/PRSS16 (2). The mouse DPP7 precursor consists of a  signal peptide (amino acids (aa) 1‑33) and a mature chain (aa 34‑506). The purified recombinant mouse DPP7 is active against Lys-Pro-AMC and Lys-Ala-AMC. Its activity against Lys-Pro-AMC is approximately 10-fold of that against Lys-Ala-AMC under otherwise identical conditions.

• References:

1. Araki, H. et al. (2001) J. Biochem. 129:279.

2. Maes, M.-B. et al. (2005) Biochem. J. 386:315.

3. Rosenblum, J.S. and J.W. Kozarich (2003) Curr. Opin. Chem. Biol. 7:496.

4. Lankas, G.R. et al. (2005) Diabetes 54:2988.

• Long Name:

  Dipeptidyl-peptidase II

• Entrez Gene IDs:

  29952 (Human); 83768 (Mouse); 83799 (Rat)

• Alternate Names:

  carboxytripeptidase; Dipeptidyl aminopeptidase II; dipeptidyl arylamidase II; dipeptidyl peptidase 2; Dipeptidyl peptidase 7; Dipeptidyl peptidase II; dipeptidylpeptidase 7; dipeptidyl-peptidase 7; dipeptidyl-peptidase II; DPP II; DPP2; DPP7; DPPII; EC 3.4.14.2; QPP; Quiescent cell proline dipeptidase