• Species Reactivity

  Mouse

• Specificity

  Detects mouse Sulfatase Modifying Factor 2/SUMF2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human (rh) SUMF2 and less than 5% cross-reactivity with rhSUMF1 is observed.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse Sulfatase Modifying Factor 2/SUMF2    
  Gln34-Leu308    
  Accession # Q8BPG6

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  0.1 µg/mL

  Recombinant Mouse Sulfatase Modifying Factor 2/SUMF2

• 
  

• Immunoprecipitation

  25 µg/mL

  Conditioned cell culture medium spiked with Recombinant Mouse Sulfatase Modifying Factor 2/SUMF2,

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Sulfatase Modifying Factor 2/SUMF2

Mouse SUMF2 is a 30 kDa member of the sulfatase-modifying factor family. It is synthesized as a 275 amino acid (aa) glycoprotein that is localized to the ER. It contains multiple calcium-binding sites and three (sub)domains of varying length and ill-defined structure (aa 38‑298). There is one potential alternate start site at aa 118 that would generate a 190 aa precursor. The founding family member, SUMF1, posttranslationally activates newly synthesized sulfatases by converting a Cysteine to formylGly in the catalytic site. SUMF2 apparently has no such activity. It forms homodimers and heterodimers with SUMF1. As a heterodimer, it downmodulates SUMF1 activity. Mature mouse SUMF2 is 85% and 93% aa identical to human and rat SUMF2, respectively.

• Entrez Gene IDs:

  25870 (Human)

• Alternate Names:

  C-alpha-formyglycine-generating enzyme 2; C-alpha-formylglycine-generating enzyme 2; DKFZp566I1024; DKFZp686I1024; DKFZp686L17160; DKFZp781L1035; FGE2; MGC99485; paralog of the formylglycine-generating enzyme; pFGE; sulfatase modifying factor 2; sulfatase-modifying factor 2; SUMF2