• Species Reactivity

  Mouse

• Specificity

  Detects mouse ASAH2/N‑acylsphingosine Amidohydrolase-2 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross‑reactivity with recombinant human ASAH2 is observed.

• Source

  Polyclonal Sheep IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse ASAH2/N‑acylsphingosine Amidohydrolase-2    
  Thr34-Thr756    
  Accession # Q9JHE3

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  0.1 µg/mL

  Recombinant Mouse ASAH2/N‑acylsphingosine Amidohydrolase-2 (Catalog # )

• 
  

• Immunoprecipitation

  25 µg/mL

  Conditioned cell culture medium spiked with Recombinant Mouse ASAH2/N‑acylsphingosine Amidohydrolase-2 (Catalog # ),

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ASAH2/N-acylsphingosine Amidohydrolase-2

The mouse ASAH2 gene encodes acylsphingosine amidohydrolase-2, also known as neutral ceramidase. Neutral ceramidase is a type II integral membrane protein that can be cleaved to produce a soluble secreted protein (1). The enzyme is abundant in the brush border membranes of the intestine, but is also expressed in tissues such as kidney, brain and liver (2, 3). A major physiological function of neutral ceramidase is the metabolism of dietary sphingolipids, but the enzyme may also be involved in the generation of messenger molecules such as sphingosine and sphingosine 1-phosphate (3).

• References:

1. Tani, M. et al. (2003) J. Biol. Chem. 278:10523.

2. Kono, M. et al. (2006) J. Biol. Chem. 281:7324.

3. Mitsutake, S. et al. (2001) J. Biol. Chem. 276:26249.

• Long Name:

  Neutral Ceramidase

• Entrez Gene IDs:

  56624 (Human); 54447 (Mouse); 114104 (Rat)

• Alternate Names:

  ASAH2 N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2; ASAH2; BCDase; HNAC1; LCDase; Nacylsphingosine Amidohydrolase2; NCDase; N-CDase