• Species Reactivity

  Mouse

• Specificity

  Detects mouse IGFBP-L1 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross‑reactivity with recombinant human IGFBP-L1 is observed and less than 1% cross-reactivity with recombinant mouse IGFBP-7 is observed.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse IGFBP-L1    
  Leu18-Leu270    
  Accession # Q80W15

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  0.1 µg/mL

  Recombinant Mouse IGFBP-L1 (Catalog # )

• 
  

• Immunohistochemistry

  5-15 µg/mL

  Immersion fixed frozen sections of embyronic mouse brain (E13.5)

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGFBP-L1

Insulin-like Growth Factor Binding Protein-Like 1 (IGFBP‑L1), also known as IGFBP-rP4, is a 38 kDa secreted member of the IGFBP superfamily of proteins. IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins (1). IGFBP-L1 contains an N-terminal IGFBP motif, a Kazal-type serine protease inhibitor region, and a C‑terminal Ig-like domain (2, 3). Mature mouse IGFBP-L1 shares 76% and 96% amino acid (aa) sequence identity with human and rat IGFBP-L1, respectively. It shares 38%-41% aa sequence identity with mouse IGFBP-7 and IGFBP-rp10 and 20%‑24% aa sequence identity with mouse IGFBP-1, -2, -3, -4, -5, and -6. IGFBP-L1 is most highly expressed in testis and brain, while lower levels are more broadly expressed (3). IGFBP-L1 is expressed in regionally and temporally distinct patterns during forebrain development (4). IGFBP-L1 inhibits cell proliferation in vitro and is downregulated in many colon and lung tumors (3). Transcriptional downregulation by promoter methylation is predictive of breast cancer aggressiveness (5).

• References:

1. Firth, S.M. and R.C. Baxter (2002) Endocr. Rev. 23:824.

2. Accession # Q80W15.

3. Cai, Z. et al. (2005) Biochem. Biophys. Res. Commun. 331:261.

4. Gonda, Y. et al. (2007) Gene Expr. Patterns 7:431.

5. Smith, P. et al. (2007) Clin. Cancer Res. 13:4061.

• Long Name:

  Insulin-like Growth Factor Binding Protein-like 1

• Entrez Gene IDs:

  347252 (Human); 75426 (Mouse)

• Alternate Names:

  bA113O24.1; IGFBPL1 insulin-like growth factor binding protein-like 1; IGFBPL1; IGFBP-L1; IGFBP-RP4