• Species Reactivity

  Mouse

• Specificity

  Detects mouse CXCL15/Lungkine in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with rm6Ckine, rmC10, rrCINC-1, rmBLC, rmCRG-2, rmEotaxin, rmFractalkine, rmGCP-2, rmJE, rmKC, rmLymphotactin, rmMARC, rmMDC, rmMIG, rmMIP-1 alpha, rmMIP-1 beta, rmRANTES, rmSDF-1 alpha, rmTCA-3 and rmTeck is observed.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  E. coli-derived recombinant mouse CXCL15/Lungkine (R&D Systems, Catalog # 442-LK)    
  Gln26-Ala167    
  Accession # Q9WVL7

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Endotoxin Level

  <0.10 EU per 1 μg of the antibody by the LAL method.  

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  0.1 µg/mL

  Recombinant Mouse CXCL15/Lungkine (Catalog # )

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL15/Lungkine

Mouse Lungkine/CXCL15 (1), also named WECHE (2), is a member of the ELR motif-containing CXC chemokines. The cDNA of mouse Lungkine encodes a protein of 166 amino acids (aa) with a 25 aa predicted signal peptide and a 141 aa mature protein with an extremely long C terminal tail that protrudes beyond the chemokine fold. Mouse Lungkine shares 35% aa sequence identity with human ENA-78 and 31% identity with human IL-8. The gene for mouse Lungkine has been mapped to chromosome 5. By Northern blot and in situ hybridization, Lungkine transcripts are only specifically detected in the adult and fetal lung, and its expression is up-regulated under inflammatory conditions (1). Lungkine protein is secreted into bronchoalveolar space and is involved in lung-specific neutrophils trafficking (1). Studies from Lungkine knock out mice suggests that Lungkine is an important mediator of neutrophil migration from the lung parenchyma into the airspace (3). Lungkine is also chemotactic for bone marrow progenitor cells and modulates hematopoietic cell differentiation (2).

• References:

1. Rossi, D.L. et al. (1999) J. Immunol. 162:5490.

2. Ohneda, O. et al. (2000) Immunity 12:141.

3. Chen, S-C. et al. (2001) J. Immunol. 166:3362.

• Entrez Gene IDs:

  20309 (Mouse)

• Alternate Names:

  CXCL15; Lungkine; Scyb15; Weche