• Species Reactivity

  Mouse

• Specificity

  Detects mouse FPRP in direct ELISAs and Western blots.

• Source

  Polyclonal Sheep IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse FPRP    
  Arg22-Pro832    
  Accession # NP_035327

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  0.1 µg/mL

  Recombinant Mouse FPRP

• 
  

• Flow Cytometry

  2.5 µg/10    ⁶ cells

  See below

• 
  

• CyTOF-ready

  Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FPRP

FPRP (F_{2 alpha} Prostaglandin receptor Regulatory Protein; also CD315, EWI-F and CD9 partner-1/P-1) is a 130 kDa type I transmembrane (TM) glycoprotein member of the EWI subfamily, Ig superfamily of molecules. It associates with the 7-TM prostaglandin F_{2 alpha}  receptor, inhibiting its activity. It also forms complexes with 4-TM CD9 and CD81, linking these molecules to the intracellular actin cytoskeleton. Mature mouse FPRP is 858 amino acids (aa) in length. Its extracellular domain (ECD) contains six C2-type Ig-like domains (aa 22‑813) plus a Pro-Thr-Glu-Leu ER retention signal. The ECD of mouse FPRP (aa 22‑832) shares 89% and 98% aa identity with the ECD of human and rat FPRP, respectively.

• Long Name:

  Prostaglandin F2-alpha Receptor Regulatory Protein

• Entrez Gene IDs:

  5738 (Human); 19221 (Mouse); 29602 (Rat)

• Alternate Names:

  CD315; CD9P-1; FPRP; PTGFRN