• Species Reactivity

  Mouse

• Specificity

  Detects mouse MESDC2 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with human MESDC2 is observed.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse MESDC2    
  Ala30-Leu224    
  Accession # Q9ERE7

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  See below

• 
  

• Immunohistochemistry

  5-15 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MESDC2

Mesoderm development candidate gene 2 (MESDC2, Mesd), also known as Boca in drosophila, is a 22 kDa protein that is required for formation of the primitive streak and mesoderm during embryogenesis (1-3). Mature mouse MESDC2 consists of an 83 amino acid (aa) structured central domain with flexible N- and C-terminal regions (4, 5). It shares 89% and 96% aa sequence identity with human and rat MESDC2, respectively. Although Boca lacks 34 aa in the C-terminal region and is only 40% identical with mouse MESDC2, the mouse protein can functionally substitute for Boca in drosophila S2 cells (6). A C-terminal ER retention motif localizes MESDC2 and Boca to the lumen of the endoplasmic reticulum (2, 6, 7). Within the ER, MESDC2 binds to the Wnt co-receptors LRP5 and LRP6 and is required for their proper folding and cell surface expression (2, 5, 8, 9). MESDC2 is therefore important for cellular Wnt responsiveness (9). When added extracellularly, MESDC2 binds to cell surface LRP6, preventing its interaction with the Wnt antagonist Dkk-1. This binding does not, however, trigger LRP6 internalization or alteration of cytoplasmic beta -catenin levels (8). An LRP5 mutant associated with high bone mass does not interact with MESDC2 (10). MESDC2 itself can be disrupted by a chromosomal translocation occurring in the germ cell tumor, infantile sacrococcygeal teratoma (11).

• References:

1. Holdener, B.C. et al. (1994) Development 120:1335.

2. Hsieh, J-C. et al. (2003) Cell 112:355.

3. Kimelman, D. (2006) Nat. Rev. Genet. 7:360.

4. Wines, M.E. et al. (2001) Genomics 72:88.

5. Koduri, V. and S.C. Blacklow (2007) Biochemistry 46:6570.

6. Culi, J. et al. (2004) EMBO J. 23:1372.

7. Culi, J. and R.S. Mann (2003) Cell 112:343.

8. Li, Y. et al. (2005) J. Cell Sci. 118:5305.

9. Li, Y. et al. (2006) FEBS Lett. 580:5423.

10. Zhang, Y. et al. (2004) Mol. Cell. Biol. 24:4677.

11. Veltman, I.M. et al. (2005) Hum. Mol. Genet. 14:1955.

• Long Name:

  Mesoderm Development Candidate 2

• Entrez Gene IDs:

  23184 (Human)

• Alternate Names:

  BOCA; KIAA0081Renal carcinoma antigen NY-REN-61; MESDC2; MESDLDLR chaperone MESD; mesoderm development candidate 2Mesoderm development protein