• Species Reactivity

  Chicken

• Specificity

  Detects chicken RGM-A in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross‑reactivity with recombinant human RGM and recombinant mouse RGM.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant chicken RGM-A    
  Trp30-Asn404 (Asn404Asp)    
  Accession # Q8JG54

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  0.1 µg/mL

  Recombinant Chicken Repulsive Guidance Molecule A/RGM-A

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RGM-A

Chicken RGM is the founding member of the RGM family of glycosylphosphatidylinositol (GPI)-anchored axon guidance proteins. It is a disulfide-linked dimer of the N-terminal RGD-containing segment (aa 30‑149) and the C-terminal segment (aa 150‑432). RGM-A binds with high affinity to neogenin. Chicken RGM-A (aa 30‑401) shares 88% and 87% aa sequence identity with its human and mouse homologs, respectively.

• Long Name:

  Repulsive Guidance Molecule A

• Entrez Gene IDs:

  56963 (Human); 244058 (Mouse)

• Alternate Names:

  RGM domain family member A; RGM domain family, member A; RGMA; RGM-A; RGMrepulsive guidance molecule A