• Species Reactivity

  Canine

• Specificity

  Detects canine RAGE in ELISAs and Western blots. In sandwich immunoassays, approximately 0.5% cross‑reactivity with recombinant human RAGE is observed and less than 0.1% cross-reactivity with recombinant mouse RAGE and recombinant rat RAGE is observed.

• Source

  Polyclonal Sheep IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant canine RAGE    
  Asp25-Val339    
  Accession # XP_532093

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  0.1 µg/mL

  Recombinant Canine RAGE (Catalog # )

• 
  

• Canine RAGE Sandwich Immunoassay

  Reagent    

• ELISA Capture (Matched Antibody Pair)

  0.2-0.8 µg/mL 

  Canine RAGE Antibody (Catalog # )

• ELISA Detection (Matched Antibody Pair)

  0.1-0.4 µg/mL 

  Canine RAGE Biotinylated Antibody (Catalog # )

• ELISA Standard

   

  Recombinant Canine RAGE Protein, CF (Catalog # )

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RAGE

Advanced glycation endproducts (AGEs) are adducts formed by the non-enzymatic glycation of macromolecules. AGE formation is accelerated in oxidative and hyperglycemic conditions, diabetes, renal failure, atherosclerosis, Alzheimer’s disease, arthritis, and in normal aging (1‑5). Receptor for advanced glycation endproducts (RAGE) is a 35 kDa type I transmembrane protein belonging the immunoglobulin superfamily. Besides AGEs, RAGE binds beta -amyloid peptide, S100/calgranulin family proteins, HMGB1/amphoterin, and leukocyte integrins (6‑9). Mature canine RAGE consists of a 383 amino acid (aa) extracellular domain (ECD) with one Ig-like V-type domain and two Ig-like C‑type domains, a 23 aa transmembrane segment, and a 43 aa cytoplasmic domain (10). Within the ECD, canine RAGE shares 73%‑77% aa sequence identity with human, mouse, and rat RAGE. In human, soluble forms of RAGE are generated by alternate splicing and are associated with multiple disease states (11, 12). RAGE is expressed in the embryonic central nervous system and on macrophages, monocytes, smooth muscle cells, and endothelial cells (13‑15). It is upregulated in response to AGE accumulation, and its activation induces a broad proinflammatory response (6, 15). The increased production of reactive oxygen species during inflammation promotes additional AGE formation and RAGE upregulation, a cycle that exacerbates diabetic complications and inflammation‑induced tissue injury (2, 4).

• References:

1. Schleicher, E. and U. Friess (2007) Kidney Int. Suppl. 106:S17.

2. Herold, K. et al. (2007) J. Leukoc. Biol. 82:204.

3. Thornalley, P.J. (2006) J. Ren. Nutr. 16:178.

4. Goldin, A. et al. (2006) Circulation 114:597.

5. Ramasamy, R. et al. (2005) Glycobiology 15:16R.

6. Kislinger, T. et al. (1999) J. Biol. Chem. 274:31740.

7. Yan, S.D. et al. (1996) Nature 382:685.

8. Huttenen, H. et al. (2000) J. Biol. Chem. 275:40096.

9. Chavakis, T. et al. (2003) J. Exp. Med. 198:1507.

10. Murua Escobar, H. et al. (2006) Gene 369:45.

11. Yonekura H, et al. (2003) Biochem. J. 370:1097.

12. Koyama, H. et al. (2007) Mol. Med. 13:625.

13. Hori, O. et al. (1995) J. Biol. Chem. 270:25752.

14. Brett, J. et al. (1993) Am. J. Pathol. 143:1699.

15. Bierhaus, A. et al. (2006) Curr. Opin. Investig. Drugs 7:985.

• Long Name:

  Receptor for Advanced Glycation End Products

• Entrez Gene IDs:

  177 (Human); 11596 (Mouse); 81722 (Rat)

• Alternate Names:

  AGER; EC 2.7.11.22; MOK protein kinase; MOKMAPK/MAK/MRK overlapping kinase; RAGE; RAGE-1; RAGE1renal cell carcinoma antigen (MOK protein kinase); Renal tumor antigen 1; renal tumor antigen