• Species Reactivity

  Rat

• Specificity

  Detects rat IL-9 R in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 40% cross-reactivity with recombinant mouse IL-9 R is observed and approximately 10% cross-reactivity with recombinant human IL-9 R is observed.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant rat IL‑9 R    
  Val38-Ala270    
  Accession # EDM04022

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  0.1 µg/mL

  Recombinant Rat IL‑9 R Subunit (Catalog # )

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-9 R

The IL-9 receptor alpha protein is a member of the type I cytokine receptor family. It is a 62 kDa IL-9 receptor subunit (previously Gfi-2, designated CD129) that binds IL-9 and pairs with the 64 kDa cytokine receptor common gamma -chain to allow cell signaling (1‑3). The 467 amino acid (aa) rat IL-9 R alpha precursor is predicted to contain a 37 aa signal sequence, a 232 aa extracellular domain (ECD) with four conserved cysteine residues in its N-terminal region, a fibronectin type III domain and a WSXWS motif, a 21 aa transmembrane (TM) domain, and a 177 aa cytoplasmic domain with a Box 1 JAK-binding motif (1, 2, 4). A potential isoform contains a 36 aa sequence that is substituted for the TM and cytoplasmic domains (5). The ECD of rat IL-9 R alpha shares 86%, 63%, 66%, 63% and 59% aa identity with the ECD of mouse, human, equine, canine and bovine IL-9 R alpha, respectively. Mast cells, germinal center and B-1b B cells, T cell blasts, and myeloid progenitors express IL-9 R (1, 6‑8). The primary functions of IL-9 have been difficult to determine due to the cross-functionality and cross-induction of cytokines. IL-9 production by regulatory T cells (T-regs), however, is crucial for mast cell recruitment and differentiation, which in turn is crucial for tolerance of allografts (7). Genetic deletion and transgenic expression of IL-9 and other cytokines has also demonstrated the role of IL-9 in mastocytosis, eosinophilia and B cell infiltration of the lung in a mouse asthma-like model, while IL-9 induction of IL-13 is responsible for eosinophil recruitment and mucous upregulation (8‑10).

• References:

1. Knoops, L. and J-C. Renauld (2004) Growth Factors 22:207.

2. Flubacher, M.M. et al. (1994) J. Virol. 68:7709.

3. Demoulin, J-B. et al. (1996) Mol. Cell. Biol. 16:4710.

4. Swissprot Accession # Q63216

5. Entrez Accession # EDM04023

6. Fawaz, L.M. et al. (2007) J. Allergy Clin. Immunol. 120:1208.

7. Lu, L-F. et al. (2006) Nature 442:997.

8. Steenwinckel, V. et al. (2007) J. Immunol. 178:3244.

9. Townsend, M.J. et al. (2000) Immunity 13:573.

10. Fallon, P.G. et al. (2002) Immunity 17:7.

• Long Name:

  Interleukin 9 Receptor

• Entrez Gene IDs:

  3581 (Human); 16199 (Mouse)

• Alternate Names:

  CD129 antigen; CD129; IL-9 R; IL-9 receptor; IL9R; IL-9R; interleukin 9 receptor; interleukin-9 receptor