• Species Reactivity

  Rat

• Specificity

  Detects rat PGD2 Synthase/PTGDS in direct ELISAs and Western blots.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant rat PGD2 Synthase/PTGDS    
  Gln25-Glu189    
  Accession # P22057

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PGD2 Synthase/PTGDS

PGD2 Synthase (prostaglandin D2 synthase; also PG-H2 D-isomerase) is a 26 kDa, monomeric member of the lipocalin family of proteins. It is expressed by neurons and astrocytes, retinal pigment epithelium and Sertoli cells. It catalyzes the conversion of PGH₂ to PGD₂, thereby influencing sleep and body temperature. It also transports retinoic acid. The rat PGD2 precursor is 189 amino acids (aa) in length. It contains a signal sequence (aa 1‑24) plus a lipocalin region that serves both as a catalytic site and hydrophobic molecule transporter (aa 40‑187). PGD2 is both intracellular and secreted. When secreted, PGD2 is called beta -Trace. One splice variant is likely intracellular and shows an 11 aa substitution for the first 52 amino acids. Over aa 25‑189, rat PGD2 shares 72% and 88% aa identity with human and mouse PGD2, respectively.

• Long Name:

  Prostaglandin D2 Synthase

• Entrez Gene IDs:

  5730 (Human); 25526 (Rat)

• Alternate Names:

  Beta-trace protein; Cerebrin-28; EC 5.3.99.2; Glutathione-independent PGD synthase; glutathione-independent PGD synthetase; lipocalin-type prostaglandin D synthase; Lipocalin-type prostaglandin-D synthase; L-PGDS; PDS; PGD2 Synthase; PGD2; PGDS2; PGDSLPGDS; PH2DISO; prostaglandin D synthase; prostaglandin D2 synthase (21kD, brain); prostaglandin D2 synthase 21kDa (brain); Prostaglandin-D2 synthase; prostaglandin-H2 D-isomerase; PTGDS