• Species Reactivity

  Rat

• Specificity

  Detects rat ALK-7 in direct ELISAs and Western blots.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant rat ALK‑7    
  Leu26-Arg108 (predicted)    
  Accession # NP_620790

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  0.1 µg/mL

  Recombinant Rat ALK‑7 Fc Chimera (Catalog # )

• 
  

• Immunohistochemistry

  5-15 µg/mL

  Perfusion fixed frozen sections of rat brain (hypothalamus)

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ALK-7

Transforming growth Factor beta (TGF-beta ) superfamily ligands exert their biological activities via binding to heteromeric receptor complexes of two types (I and II) of serine/threonine kinases. Type II receptors are constitutively active kinases that phosphorylate type I receptors upon ligand binding. In turn, activated type I kinases phosphorylate downstream signaling molecules including the various smads. Transmembrane proteoglycans, including the type III receptor (betaglycan) and endoglin, can bind and present some of the TGF-beta superfamily ligands to type I and II receptor complexes and enhance their cellular responses. Seven type I receptors (also termed activin receptor-like kinase (ALK)) and five type II receptors have been isolated from mammals. ALK-2, -3, -4, -5, and -6 are also known as Activin R1A, BMPR-1A, Activin R1B, TGF-beta R1, and BMPR-1B, respectively, reflecting their ligand preferences. ALK-7 shares with other type I receptors a cysteine-rich domain with conserved cysteine spacing in the extracellular region, and a glycine-and serine-rich domain (the GS domain) preceding the kinase domain. Rat ALK-7 expression is restricted to the adult central nervous system, suggesting a possible role of ALK-7 during postnatal maturation and maintenance of several neuronal subpopulations in the cerebellum.

• References:

1. ten Dijke, P. et al. (1993) Oncogene 8:2879.

2. ten Dijke, P. et al. (1994) Science 264:101.

3. Ryden, M. et al. (1996) J. Biol. Chem. 271:30603.

• Long Name:

  Activin Receptor-like Kinase 7

• Entrez Gene IDs:

  130399 (Human); 245921 (Rat)

• Alternate Names:

  activin A receptor, type IC; Activin receptor type IC; activin receptor type-1C; Activin receptor-like kinase 7; Activin RIC; ACTR-IC; ACVR1C; ACVRLK7; ALK7; ALK-7; ALK7ALK-7; EC 2.7.11; EC 2.7.11.30