详细说明
Species Reactivity
Human, Mouse
Specificity
Detects mouse and human Cadherin-17 in direct ELISAs and Western blots. In direct ELISAs, less then 10% cross-reactivity with recombinant human Cadherin-17 is observed.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Cadherin-17
Glu22-Met786
Accession # Q9R100Formulation
Supplied as a 0.2 μm filtered solution in PBS containing BSA, Glycerol and Sodium Azide.
Label
Unconjugated
Applications
Recommended
ConcentrationSample
Western Blot
1:1000 dilution
See below
Simple Western
1:100 dilution
See below
Immunohistochemistry
1:250 dilution
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Western Blot | Detection of Human and Mouse Cadherin‑17 by Western Blot. Western blot shows lysates of human colon tissue and mouse colon tissue. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Human/Mouse Cadherin‑17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8524) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # ). A specific band was detected for Cadherin‑17 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using . |
Immunohistochemistry | Cadherin‑17 in Mouse Intestine. Cadherin‑17 was detected in perfusion fixed frozen sections of mouse intestine using Rabbit Anti-Mouse Cadherin‑17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8524) at 1:250 dilution overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # ) and counterstained with DAPI (blue). Specific staining was localized to the plasma membrane of glandular cells. View our protocol for . |
Simple Western | Detection of Human and mouse Cadherin‑17 by Simple WesternTM. Simple Western lane view shows lysates of human colon tissue and mouse colon tissue, loaded at 0.2 mg/mL. Specific bands were detected for Cadherin‑17 at approximately 152 and 146 kDa (as indicated) using 1:100 dilution of Rabbit Anti-Human/Mouse Cadherin‑17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8524). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C, as supplied.
1 month, 2 to 8 °C under sterile conditions after opening.
6 months, -20 to -70 °C under sterile conditions after opening.
Background: Cadherin-17
Cadherin-17, also known as liver-intestine (LI) Cadherin, belongs to the cadherin family of calcium-dependent cell adhesion molecules. It associates homotypically and is selectively expressed on gastric and intestinal mucosa.
Entrez Gene IDs:
1015 (Human); 12557 (Mouse)
Alternate Names:
cadherin 17, LI cadherin (liver-intestine); cadherin; cadherin-16; Cadherin17; Cadherin-17; CDH16; CDH17; FLJ26931; HPT-1 cadherin; HPT1; HPT-1; human intestinal peptide-associated transporter HPT-1; human peptide transporter 1; Intestinal peptide-associated transporter HPT-1; LI cadherin; LI-cadherin; Liver-intestine cadherin; MGC138218; MGC142024
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