• Purity

  >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability to induce IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. Yao, Z.     et al. (1995) Immunity     3:811. The ED    ₅₀ for this effect is 3-18 ng/mL.

• Source

  Chinese Hamster Ovary cell line, CHO-derived

  Rat IL-17A (Ala18-Ser150 (Ile46Leu)) Accession # Q61453

  Rat IL-17F (Arg29-Ala161) Accession # Q5BJ95
  N-terminus	C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  Ala18 (IL-17A) & Arg29 (IL-17F)

• Structure / Form

  Disulfide-linked heterodimer

• Predicted Molecular Mass

  15 kDa (IL-17A) & 15 kDa (IL-17F)

• SDS-PAGE

  12-30 kDa, reducing conditions

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Data Images

Background: IL-17A/F Heterodimer

Rat IL-17A/F is an approximately 37 kDa, secreted, disulfide-linked glycosylated heterodimeric protein comprised of two members of the IL-17 family of cytokines, IL-17A and IL-17F (1, 2). Members of this family demonstrate a structural motif termed a cysteine knot that also characterizes a large superfamily of growth factors such as TGF-beta (1). Although most cysteine knot superfamily members use three intrachain disulfide bonds to create a knot, IL-17 family molecules generate the same structural form with only two disulfide links (1, 3-5). Mature rat IL-17A and IL-17F share 60% and 59% amino acid sequence identity with human and 61% and 90% with mouse IL-17A and IL-17F, respectively. They also share 50% aa sequence identity with each other (1). IL-17A/F heterodimer and the IL-17A and IL-17F homodimers are produced by IL-23 activated Th17 cells (1, 6-10, 14). The widely expressed receptors IL-17 RA and IL-17 RC form a heterodimer for the binding of  
IL-17A and IL-17F, as well as the heterodimeric IL-17A/F (6, 11, 12). IL-17A/F is a biologically active protein that induces chemokine production and airway neutrophilia with intermediate potency between IL-17A (most potent) and IL-17F (least potent) (7, 12). It is up-regulated in immune cells during inflammatory arthritis and contributes to disease severity (13).

• References:

1. Wright, J.F. et al. (2007) J. Biol. Chem. 282:13447.

2. Chang, S.H. and C. Dong (2007) Cell Res. 17:435.

3. Kawaguchi, M. et al. (2004) J. Allergy Clin. Immunol. 114:1265.

4. Kolls, J.K. and A. Linden (2004) Immunity 21:467.

5. Gu, C. et al. (2013) Cytokine 64:477.

6. Cheung, P.F.Y. et al. (2008) J. Immunol. 180:5625.

7. Liang, S.C. et al. (2007) J. Immunol. 179:7791.

8. Ouyang, W. et al. (2008) Immunity 28:454.

9. Steinman, L. (2007) Nat. Med. 13:139.

10. Hunter, C.A. (2005) Nat. Rev. Immunol. 5:521.

11. Kuestner, R.E. et al. (2007) J. Immunol. 179:5462.

12. Wright, J.F. et al. (2008) J. Immunol. 181:2799.

13. Sarkar, S. et al. (2014) Clin. Exp. Immunol. 177:652.

14. Spolski, R. et al. (2009) Eur. J. Immunol. 3: 658.

• Long Name:

  Interleukin 17 A/F

• Alternate Names:

  CTLA8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; Cytotoxic T-Lymphocyte-Associated Protein 8; IL17; IL-17; IL-17A; IL-17A/F Heterodimer; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A; interleukin-17A