详细说明
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit Type-I and Type-III IFN-mediated anti-viral activity. Huang, J. et al. (2007) PANS 104:9822. The ED 50 for this effect is 2-12 ng/mL in the presence of 20 pg/mL Recombinant Human IFN-beta (Catalog # ).
Source
Chinese Hamster Ovary cell line, CHO-derived
Viral 136R/Y136
(Tyr17-Lys351)
Accession # Q9DHH7HPGGGSGGGSGGGS HHHHHH N-terminus C-terminus N-terminal Sequence
AnalysisTyr17
Predicted Molecular Mass
41 kDa
SDS-PAGE
88-120 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
8976-BR/CF |
| 8976-BR |
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. | Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. | |
Reconstitution Reconstitute at 500 μg/mL in PBS. | Reconstitution Reconstitute at 500 μg/mL in PBS. | |
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
| Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: 136R
136R, also known as Y136, is an approximately 80 kDa glycosylated and secreted protein encoded by the Yaba-like disease virus genome (1). 136R binds directly to type I and type III interferons (IFN-alpha and IFN-lambda) (1). It prevents the interaction of rhesus and human IFN-alpha as well as human IFN-beta with the IFN-gamma R1 complex, although it shares only 14% amino acid sequence identity with the ECD of human IFN-gamma R1 (1). 136R blocks STAT1 signaling and the anti-viral protective functions of IFN-alpha, -beta, -omega, -kappa, and -lambda 1/IL-29 (1).
References:
Huang, J. et al. (2007) Proc. Natl. Acad. Sci. USA 104:9822.
Long Name:
Soluble Type I Interferon Receptor 136R
Alternate Names:
136R
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