详细说明
- Species ReactivityHuman
- SpecificityDetects human IL-27/IL-35 EBI3 Subunit in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) IL-27 EBI3 or rmIL-27 (EBI3 + p28 heterodimer) is observed.
- SourceMonoclonal Mouse IgG1 Clone # 607201
- ImmunogenMouse myeloma cell line NS0-derived recombinant human IL-27/IL-35 EBI3 Subunit
Arg21-Lys229 (predicted)
Accession # Q14213 - FormulationSupplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
- LabelAlexa Fluor 647
Applications
- Recommended
ConcentrationSample
- Intracellular Staining by Flow Cytometry0.25-1 µg/10 6 cellsKG-1 human acute myelogenous leukemia cell line treated with Recombinant Human IL-18/IL-1F4 (Catalog # ), fixed with paraformaldehyde and permeabilized with saponin
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Preparation and Storage
- ShippingThe product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
- Stability & StorageStore the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-27/IL-35 EBI3 Subunit
IL-27 is a heterodimeric cytokine comprised of the IL-12 p35-related protein, p28, and the IL-12 p40-related protein, EBI3 (Epstein-Barr virus-induced gene 3). IL-27 is expressed by monocytes, endothelial cells and dendritic cells. It binds TCCR/WSX-1 on naive CD4+ T cells and induces the expression of a functional IL-12 receptor, making these cells sensitive to IL-12-mediated Th1 cell development. Human EBI3 also associates with the p35 subunit of IL-12 to form IL-35 which is important for immunosuppressive Treg cell induction. EBI3 is 61% amino acid (aa) identical to mouse EBI3 and includes an 20 aa signal peptide anda 209 aa mature protein with two fibronectin type III domains.
- Long Name:Epstein-Barr virus induced gene 3
- Entrez Gene IDs:10148 (Human); 50498 (Mouse); 680609 (Rat); 102144621 (Cynomolgus Monkey)
- Alternate Names:cytokine receptor; EBI3; EBV-induced gene 3 protein; Epstein-Barr virus induced 3; Epstein-Barr virus induced gene 3; Epstein-Barr virus-induced gene 3 protein; IL-27 subunit beta; IL-27/IL-35 EBI3 Subunit; IL27B; IL-27B; IL35 EBI3; interleukin-27 subunit beta
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