• Species Reactivity

  Mouse

• Specificity

  Detects mouse METRNL in direct ELISAs.    
   

• Source

  Monoclonal Rat IgG2b Clone # 829535

• Immunogen

  Chinese hamster ovary cell line CHO-derived recombinant mouse METRNL    
  Gln46-Glu311    
  Accession # Q8VE43

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 488

Applications

• Recommended    
  Concentration

  Sample

• Intracellular Staining by Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  Neuro-2A mouse neuroblastoma cell line

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Meteorin-like/METRNL

Meteorin-like protein (METRNL) is a 311 amino acid (aa) protein that shows 45% homology with Meteorin (in mouse). Meteorin itself is a secreted 30 kDa protein that is expressed in glial and neuronal cells and their precursors during development and in the adult (1‑3). In the adult Meteorin is primarily expressed by astrocytes (3). Meteorin promotes neurite outgrowth, glial cell differentiation, and neuronal survival following excitotoxic injury (2‑5). It also inhibits sprouting angiogenesis and promotes blood vessel maturation (1). Mouse Meteorin‑like protein is synthesized with a 45 amino acid (aa) signal peptide and is selectively expressed in the otic vesicle during inner ear development (6, 7). Mature mouse Meteorin‑like protein shares 79% and 98% aa sequence identity with human and rat Meteorin‑like protein, respectively. Alternate splicing generates an additional isoform that lacks the N‑terminal 82 amino acids including the signal peptide. R&D Systems in‑house testing indicates that Meteorin‑like protein enhances the outgrowth of neurites from cultured mouse cortical neurons. The promoter region of the METRNL gene contains a recognition site for the Pax2, Pax5, and Pax8 transcription factors, and Pax2 binds specifically to this region (7). METRNL is the only gene found within the terminus of the human chromosome 17 q‑arm which can be deleted in the rare Ring 17 syndrome (8).

• References:

1. Park, J.A. et al. (2008) Glia 56:247.

2. Jorgensen, J.R. et al. (2009) J. Mol. Neurosci. 39:104.

3. Lee, H.S. et al. (2010) J. Cell Sci. 123:1959.

4. Nishino, J. et al. (2004) EMBO J. 23:1998.

5. Jorgensen, J.R. et al. (2011) Neurobiol. Dis. 41:160.

6. Accession # Q8VE43.

7. Ramialison, M. et al. (2008) Genome Biol. 9:R145.

8. Surace, C. et al. (2009) Clin. Genet. 76:256.

• Entrez Gene IDs:

  284207 (Human); 210029 (Mouse); 316842 (Rat)

• Alternate Names:

  Meteorin like; meteorin, glial cell differentiation regulator-like; meteorin-like protein; Meteorin-like; METRNL; MGC99788