• Species Reactivity

  Human

• Specificity

  Detects human LDHB in direct ELISAs and Western blots.    
   

• Source

  Monoclonal Rabbit IgG Clone # 2057D

• Immunogen

  E. coli-derived recombinant human LDHB    
  Met1-Val93    
  Accession # P07195

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 647

Applications

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  Recommended    
  Concentration

  Sample

• Intracellular Staining by Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  HeLa human cervical epithelial carcinoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # ) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # )

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Lactate Dehydrogenase B/LDHB

LDHB is one of two subunits of Lactose Dehydrogenase which is formed from tetramer arrangements of the A and B subunits.  LDHB is part of the pyruvate fermentation to lactate pathway in which it catalyzes the conversion of L-lactate and NAD to pyruvate and NADH.  LDHA is widely expressed, with high expression in muscle and brain.  High expression in tumor cells correlates with low survival rates in various cancers including osteosarcoma, oral squamous cell carcinoma breast and lung cancers. Unlike Lactose Dehyrogenase A, LDHB mutations are not linked to any disease

• Entrez Gene IDs:

  3945 (Human); 16832 (Mouse); 24534 (Rat)

• Alternate Names:

  EC 1.1.1; EC 1.1.1.27; Epididymis Secretory Protein Li 281; HEL-S-281; Lactate Dehydrogenase B; LDH heart subunit; LDHB; LDH-B; LDHBD; LDH-H; L-lactate dehydrogenase B chain; Renal Carcinoma Antigen NY-REN-46; TRG-5