• Species Reactivity

  Mouse

• Specificity

  Detects mouse IL‑17 RB in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-17 RB, rhIL-17 R,  or recombinant mouse IL-17 is observed.

• Source

  Monoclonal Rat IgG1 Clone # 752101

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse IL‑17 RB    
  Arg18-Gly286    
  Accession # Q9JIP3

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 350

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  HEK293 human embryonic kidney cell line transfected with mouse IL-17 RB

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-17 RB

The Interleukin 17 (IL-17) family of cytokines, comprising six members (IL-17, IL-17B through IL-17F), are structurally related proteins with a conserved cysteine-knot structure. These proinflammatory cytokines can induce local cytokine production and are involved in the regulation of the immune response. The cognate receptors activated by some of these cytokines have been identified (1, 2). Interleukin-17B Receptor (IL-17 RB), also known as IL-17 Rh1, IL-17E R and EVI27, represents the second receptor of the IL-17 family cytokines to be recognized (2‑4). Mouse IL-17 RB cDNA encodes a 499 amino acid (aa) type I membrane protein with a putative 17 aa signal peptide, a 269 aa extracellular domain, a 21 aa transmembrane domain and a 192 aa cytoplasmic tail. As a result of alternative splicing, a secreted variant of IL-17 RB also exists (5). Human and mouse IL‑17 RB share 76% aa sequence identity. IL-17 RB is approximately 20% identical to the human and mouse IL‑17 R. However, the receptors share many conserved cysteine residues within their extracellular domains as well as additional conserved elements within their cytoplasmic domains. At least three additional type I transmembrane receptors with homology to IL‑17 R, including IL-17 RL (IL-17 RC), IL-17 RD, and IL-17 RE, have been reported (2, 6). By Northern blot analysis, mouse IL-17 RB is highly expressed in liver and testes and is expressed at lower levels in kidney and lung. It is also expressed in some hematopoietic cell lines, including selected T cell, B cell, and myeloid cell lines (2‑4). The expression of IL-17 RB is significantly upregulated under inflammatory conditions (7). IL-17 RB binds strongly to IL-17E and weakly to IL-17B. It does not bind IL-17 or IL-17F. Activation of IL-17 RB by its ligands results in the activation of nuclear factor kappa-B (2‑4).

• References:

1. Aggarwal, S. and A.L. Gurney (2002) J. Leukoc. Biol. 71:1.

2. Moseley, T.A. et al. (2003) Cytokine & Growth Factor Rev. 14:155.

3. Shi, Y. et al. (2000) J. Biol. Chem. 275:19167.

4. Lee, J. et al. (2001) J. Biol. Chem. 276:1660.

5. Tian, E. et al. (2000) Oncogene 19:2098.

6. Haudenschild, D. et al. (2002) J. Biol. Chem. 277:4309.

7. Hurst, S.D. et al. (2002) J. Immunol. 169:443.

• Long Name:

  Interleukin 17B Receptor

• Entrez Gene IDs:

  55540 (Human); 50905 (Mouse)

• Alternate Names:

  CRL4IL-17 receptor homolog 1; cytokine receptor CRL4; Cytokine receptor-like 4; Evi27; EVI27IL-17B receptor; IL-17 RB; IL-17 receptor B; IL-17 RH1; IL17BRinterleukin 17B receptor; IL-17ER; IL17RB; IL-17RB; IL17Rh1; IL-17Rh1; IL17RH1MGC5245; interleukin 17 receptor B; interleukin 17 receptor homolog 1; interleukin-17 receptor B; Interleukin-17B receptor