• Purity

  >90%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured in a competitive binding assay. When rrNeurexin-1 alpha (Catalog # 4485-NX) is immobilized at 1 µg/mL (100 µL/well), rhNeurexophilin-1 inhibits 50% binding of biotinylated rrNeurexophilin-1 (250 ng/mL) at the concentration range of 1‑3 µg/mL.

• Source

  Mouse myeloma cell line, NS0-derived Ala22-Gly271, with a C-terminal 6-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  Ala22

• Predicted Molecular Mass

  29.4 kDa

• SDS-PAGE

  40-60 kDa, reducing conditions

Background: Neurexophilin-1

Neurexophilin-1 (NXPH1) is one of at least four vertebrate neuropeptide-like secreted glycoproteins in the neurexophilin family (1, 2). The 271 amino acid (aa) NXPH1 sequence contains a 21 aa signal peptide, an 86 aa propeptide that is cleaved at a basic motif, and a 164 aa mature protein that contains three potential N-glycosylation sites in the N-terminal portion and six conserved cysteines in the C-terminal portion (1). Mature human NXPH1 shares 99% aa identity with mouse, rat, equine, bovine and canine NXPH1. NXPH1 is expressed selectively in subpopulations of neurons within the cerebral cortex, cerebellum and olfactory bulb that are thought to be inhibitory interneurons (2, 3). In humans, NXPH-2 and -3 are most similar to NXPH1, sharing 84% and 64% aa identity within the mature region, respectively. In rodents, which do not express NXPH-2, expression of NXPH1 and NXPH-3 does not appear to coincide. However, both serve as tightly bound extracellular ligands for alpha -neurexins, synaptic transmembrane molecules that are essential for calcium-triggered neurotransmitter release (1, 4, 5). By contrast, NXPH-4 does not bind alpha -neurexins (1, 4). Genetic deletion of NXPH1 and/or NXPH-3 produces no anatomical effect, although mice lacking NXPH-3 show defects in motor coordination (4, 6).

• References:

1. Missler, M. et al. (1998) J. Neurosci. 18:3630.

2. Petrenko, A. G. et al. (1996) J. Neurosci. 16:4360.

3. Clarris, H. J. et al. (2002) Int. J. Dev. Biol. 46:649.

4. Missler, M. et al. (1998) J. Biol. Chem. 273:34716.

5. Dudanova, I. et al. (2006) J. Neurosci. 26:10599.

6. Beglopoulos, V. et al. (2005) Mol. Cell. Biol. 25:7278.

• Entrez Gene IDs:

  30010 (Human); 18231 (Mouse); 25501 (Rat)

• Alternate Names:

  neurexophilin 1; Neurexophilin1; Neurexophilin-1; NPH1; NXPH1