详细说明
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to bind biotinylated rhEphrin-A3/Fc Chimera in a functional ELISA.
Source
Mouse myeloma cell line, NS0-derived
Human EphA10
(Glu34 - Ala565)
Accession # NP_001092909IEGRMD Human IgG1
(Pro100 - Lys330)N-terminus C-terminus Accession #
N-terminal Sequence
AnalysisGlu34
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
84.3 kDa (monomer)
SDS-PAGE
100-106 kDa, reducing conditions
5188-EA |
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Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | ||
Reconstitution Reconstitute at 200 μg/mL in sterile PBS. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: EphA10
EphA10 is a member of the transmembrane Eph receptor tyrosine kinase family. Although the A and B classes of Eph proteins have a common structural organization, they are distinguished by their preference for Ephrin ligands. Eph-Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression (1 - 4). The 532 amino acid (aa) extracellular domain (ECD) of human EphA10 contains an N-terminal Ephrin binding region followed by two fibronectin type II domains, a 422 aa cytoplasmic domain contains the tyrosine kinase domain and a sterile alpha motif (SAM) (5). Alternate splicing generates a soluble isoform that is truncated following the Ephrin binding region, and a transmembrane isoform that lacks only the SAM (5). Within the ECD, human EphA10 shares 38% - 54% aa sequence identity with other EphA and EphB proteins. It shares 95% and 88% aa sequence identity with the ECD of mouse and rat EphA10, respectively. EphA10 is preferentially expressed in testis and is upregulated in some breast and prostate carcinoma cell lines (5 - 7). It selectivity binds Ephrin-A1, 2, 3, 4,and 5 relative to Ephrin-B1 and -B2 (5).
References:
Pasquale, E.B. (2008) Cell 133:38.
Merlos-Suarez, A. and E. Batlle (2008) Curr. Opin. Cell Biol. 20:194.
Arvanitis, D. and A. Davy (2008) Genes Dev. 22:416.
Robinson, D.R. et al. (2000) Oncogene 19:5548.
Aasheim, H.-C. et al. (2005) Biochim. Biophys. Acta 1723:1.
Fox, B.P. et al. (2006) Biochem. Biophys. Res. Commun. 342:1263.
Fox, B.P. and R.P. Kandpal (2004) Biochem. Biophys. Res. Commun. 318:882.
Long Name:
Eph Receptor A10
Entrez Gene IDs:
284656 (Human); 298528 (Rat)
Alternate Names:
EC 2.7.10.1; EPH receptor A10; EphA10; EphA10s protein; ephrin type-A receptor 10; FLJ16103; FLJ33655; MGC43817