• Purity

  >90%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <0.01 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its binding ability in a functional ELISA. Biotinylated rrNLGN-1v2 immobilized on a streptavidin-coated plate at 2 µg/mL    
  (100 μL/well) can bind rhNeurexin 3 beta /Fc Chimera with an apparent K    _D <40 nM.  

• Source

  Mouse myeloma cell line, NS0-derived

  Human Neurexin 3 beta (Ser35 - Thr357) Accession # NP_620426	IEGRMD	Human IgG1 (Pro100 - Lys330)
  N-terminus		C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  Ser35

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  61.1 kDa (monomer)

• SDS-PAGE

  85-96 kDa, reducing conditions

Background: Neurexin 3 beta/NRXN3b

The alpha and beta forms of Neurexins 1 - 3 are transmembrane neuronal glycoproteins which are transcribed from each of three NRXN genes that utilize alternate promoters. Like other Neurexins, the extracellular domain (ECD) of Neurexin 3 alpha contains six LNS domains interspersed with three EGF-like domains, while that of Neurexin 3 beta contains only the sixth LNS domain and no EGF-like domains (1 - 3). Mature human Neurexin 3 beta is a 70 kDa glycosylated protein with a 528 amino acid (aa) ECD and a 56 aa cytoplasmic domain that contains a motif for binding PDZ scaffolding proteins (3 - 5). Within comparable regions of the ECD, human Neurexin 3 beta shares 99% aa sequence identity with mouse and rat Neurexin 3 beta. It shares 65% aa sequence identity with comparable regions of the ECD of human Neurexin 1 beta and 2 beta. Alternative splicing of human Neurexin 3 beta generates multiple isoforms. There are potentially soluble and secreted variants and some which contain a fibronectin type III-like domain (4, 6). Neurexin 3 beta is widely expressed in the brain where it binds the postsynaptic Neuroligins 1, 2, and 3 (6 - 9). Neurexin 3 beta may also be expressed in non-nervous tissues with a potentially cardiac-specific isoform (10). Human Neurexin 3 beta polymorphisms which affect the splicing pattern are associated with susceptibility to alcohol dependence (6). The Neurexin 3 beta genetic locus has been linked to opioid and nicotine addiction, and Neurexin 3 beta gene expression is upregulated after short term exposure of mice to cocaine (11 - 13).

• References:

1. Craig, A.M. and Y. Kang (2007) Curr. Opin. Neurobiol. 17:43.

2. Dean, C. and T. Dresbach (2006) Trends Neurosci. 29:21.

3. Lise, M.-F. and A. El-Husseini (2006) Cell. Mol. Life Sci. 63:1833.

4. Rowen, L. et al. (2002) Genomics 79:587.

5. Ushkaryov, Y. A. et al. (1994) J. Biol. Chem. 269:11987.

6. Hishimoto, A. et al. (2007) Hum. Mol. Genet. 16:2880.

7. Ullrich, B. et al. (1995) Neuron 14:497.

8. Ichtchenko, K. et al. (1996) J. Biol. Chem. 271:2676.

9. Ichtchenko, K. et al. (1995) Cell 81:435.

10. Occhi, G. et al. (2002) Biochem. Biophys. Res. Commun. 298:151.

11. Lachman, H.M. et al. (2007) Hum. Mol. Genet. 16:1327.

12. Bierut, L.J. et al. (2007) Hum. Mol. Genet. 16:24.

13. Kelai, S. et al. (2008) Neuroreport 19:751.

• Entrez Gene IDs:

  9369 (Human); 18191 (Mouse)

• Alternate Names:

  Neurexin 3 beta