• Purity

  >95%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <0.01 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Optimal neurite outgrowth was observed when neurons were plated on 96-well culture plates that were pre-coated with 50 μL/well of rhKiron at 10-50 μg/mL.

• Source

  Mouse myeloma cell line, NS0-derived Val38-Gly324, with a C-terminal 6-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  Val38

• Predicted Molecular Mass

  32.2 kDa

• SDS-PAGE

  43-60 kDa, reducing conditions

Background: Kilon/NEGR1

Kindred of IgLON (Kilon; also neuronal growth regulator 1 (negr1) and neurotractin) is a 46 kDa member of the IgLON family of molecules. This cell adhesion family includes the proteins LAMP, OBCAM, neurotrimin, CEPU-1, AvGP50, and GP55 (1). Human Kilon is synthesized as a 354 amino acid (aa) precursor that contains a 37 aa signal sequence, a 287 aa mature chain, and a 30 aa propeptide. The mature chain consists of three C2 Ig-like domains, six potential sites for N-linked glycosylation, and a GPI anchor. In addition, there are three sets of cysteines that have the potential to form intradomain disulfide linkages in each of the mature chain's Ig-like domains (1). Human Kilon shares 97% aa sequence identity with mouse and rat Kilon. Expression of Kilon is restricted to the brain, specifically in the cerebrum, brain stem, and hippocampus, with much less expression in the cerebellum (1). In the rat, it was shown that Kilon is already expressed in the E16 stage, and its level gradually increases during development (1). In the cerebral cortex, numerous puncta of Kilon immunoreactivity were visible in all regions, and most were densely distributed in large neurons of layer V (1). These neurons were identified as pyramidal neurons because of their soma location in layer V, large soma size, and extension of their apical dendrite to layer I (1). Kilon may be involved in cell-adhesion and may function as a trans-neural growth-promoting factor in regenerative axon sprouting in the mammalian brain (1 - 2).

• References:

1. Funatsu, N. et al. (1999) J. Biol. Chem. 274:8224.

2. Marg, A. et al. (1999) J. Cell Biol. 145:865.

• Long Name:

  A Kindred of IgLON /Neuronal Growth Regulator 1

• Entrez Gene IDs:

  257194 (Human); 320840 (Mouse); 59318 (Rat)

• Alternate Names:

  a kindred of IgLON; IgLON family member 4; IGLON4DMML2433; Kilon; KILONMGC46680; NEGR1; neuronal growth regulator 1; Neurotractin; Ntra