• Purity

  >90%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by the ability of the immobilized protein to support the adhesion of U‑87 MG human glioblastoma/astrocytoma cells. When 5 x 10    ⁴ cells per well are added to rhGPR-115 coated plates (5 μg/mL, 100 μL/well), approximately 60-85% will adhere after 60 minutes at 37 °C.

• Source

  Chinese Hamster Ovary cell line, CHO-derived Ser22-Ala347, with a C-terminal 6-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  Ser22

• Predicted Molecular Mass

  37 kDa

• SDS-PAGE

  66-100 kDa, reducing conditions

Background: GPR115

GPR115 is a member of the LN-7TM family of adhesion-type 7-transmembrane (TM) G-protein coupled receptors (GPCR) that show a long extracellular N-terminus (1, 2). The 695 amino acid (aa) human GPR115 sequence predicts a 21 aa signal sequence, a 385 aa N-terminal extracellular domain (ECD), seven TM regions separated by 6 - 24 aa intracellular and extracellular regions, and a 40 aa cytoplasmic tail. Like other LN-7TM members, the ECD contains a highly glycosylated mucin-like stalk that is predicted to function in adhesion. This is followed by a cysteine-rich GPCR proteolytic cleavage site (GPS) (1). GPS domains, which have been described in other 7TM proteins including ETL, GPR126, HE6, and Latrophilin-1, are cleavage sites for processing proteins into two subunits (3 - 7). Within the N terminal region that ends with the predicted cleavage site (aa 22 - 347), human GPR115 shares 58% aa sequence identity with the corresponding region of mouse and rat GPR115. GPR115 was identified from expressed sequence tags (ESTs) found in pregnant uterus, breast, and the genitourinary tract (1).

• References:

1. Fredriksson, R. et al. (2002) FEBS Lett. 531:407.

2. Bjarnadottir, T.K. et al. (2004) Genomics 84:23.

3. Nechiporuk, T. et al. (2001) J. Biol. Chem. 276:4150.

4. Moriguchi, T. et al. (2004) Genes Cells 9:549.

5. Kierszenbaum, A.L. (2003) Mol. Reprod. Dev. 64:1.

6. Krasnoperov, V.G. et al. (1997) Neuron 18:925.

7. Krasnoperov, V. et al. (2002) J. Biol. Chem. 277:46518.

• Long Name:

  G Protein-coupled Receptor 115

• Entrez Gene IDs:

  221393 (Human); 78249 (Mouse); 501106 (Rat)

• Alternate Names:

  FLJ38076; G protein-coupled receptor 115; GPR115; G-protein coupled receptor PGR18; PGR18; PGR18G-protein coupled receptor 115; probable G-protein coupled receptor 115; seven transmembrane helix receptor