详细说明
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Optimal neurite outgrowth was observed when neurons were plated on 96-well culture plates that had been pre-coated with 50 µL/well of the recombinant human Contactin-2 solution at 6-24 μg/mL.
Source
Mouse myeloma cell line, NS0-derived Glu20-Pro992, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
AnalysisGlu20
Predicted Molecular Mass
108.1 kDa
SDS-PAGE
140-150 kDa, reducing conditions
5539-CN |
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Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | ||
Reconstitution Reconstitute at 300 μg/mL in PBS. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: Contactin-3
Contactin-3, also known as BIG-1 and PANG, is a 150 kDa member of the TAG/F3 subgroup of Contactin neuronal adhesion proteins (1, 2). Mature human Contactin-3 consists of six immunoglobulin-like domains, a flexible linker region, four fibronectin type III domains, and a GPI anchor (3, 4). Human Contactin-3 shares 92% aa sequence identity with mouse and rat Contactin-3. Contactin-3 is primarily expressed in the brain in discrete regions including the frontal lobe, occipital lobe, cerebellum, amygdala, and hippocampus (5, 6). Alternative splicing in rat may generate a soluble isoform (4). Mouse plasmacytoma cell lines produce multiple transcripts of Contactin-3 due to the presence of IAP proviruses (3). Immobilized Contactin-3 promotes neurite outgrowth from rat hippocampal neurons (4).
References:
Shimoda, Y. and K. Watanabe (2009) Cell Adhesion Migration 3:64.
Denisenko-Nehrbass, N. et al. (2002) J. Physiol. 96:99.
Connelly, M.A. et al. (1994) Proc. Natl. Acad. Sci. 91:1337.
Yoshihara, Y. et al. (1994) Neuron 13:415.
Kamei, Y. et al. (2000) Genomics 69:113.
Yoshihara, Y. et al. (1995) J. Neurobiol. 28:51.
Entrez Gene IDs:
5067 (Human); 18488 (Mouse); 54279 (Rat)
Alternate Names:
BIG-1; CNTN3; contactin 3 (plasmacytoma associated); Contactin3; Contactin-3; PANG; PCS
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