• Purity

  >95%, by SDS-PAGE with silver staining

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability to induce cell clumping and inhibit neurite outgrowth of Neuro‑2A mouse neuroblastoma cells. 1 μg/mL of Recombinant Human SEZ6L is able to signifcantly induce cell clumping and inhibit neurite outgrowth.

• Source

  Chinese Hamster Ovary cell line, CHO-derived Leu29-Ala958, with a C-terminal 6-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  Leu29

• Predicted Molecular Mass

  102 kDa

• SDS-PAGE

  130-200 kDa, reducing conditions

Background: SEZ6L

Seizure 6-like Protein (SEZ6L) is a member of the SEZ6 family of proteins. It has a predicted molecular weight of approximately 110 kDa; however, human SEZ6L contains 11 potential N-linked glycosylation sites, and native mouse BSRP/SEZ6L is approximately 200 kDa (1, 2). Alternative splicing of the human   Sez6l gene may generate at least ten isoforms (2). Human SEZ6L is a type I transmembrane glycoprotein composed of an extracellular domain (ECD) that contains two CUB domains and five short consensus repeats (SCRs) (2). It shares 80% and 81% amino acid (aa) sequence identity with the mouse and rat orthologs, respectively. SEZ6L is expressed in the plasma membrane and endoplasmic reticulum of neurons and lung bronchiolar epithelium and likely regulates PKC alpha activation (1, 2). Human SEZ6L has been shown to be involved in the maintenance of cellular cholesterol homeostasis (3). Additionally, in mouse pancreatic beta  cells, SEZ6L has been shown to be a substrate for BACE-2, which cleaves its ECD at a site 16 aa upstream from its transmembrane domain (4). BSRP-C, also known as SEZ6, has been reported to inhibit neurite outgrowth in mice (5). R&D Systems in-house testing shows that human SEZ6L also exhibits this function. Genetic variations in human   Sez6l have been associated with bipolar disorder I and an increased risk for lung cancer (2, 6, 7).

• References:

1. Miyazaki, T. et al. (2006) FEBS Lett. 580:4057.

2. Nishioka, M. et al. (2000) Oncogene 19:6251.

3. Blattmann, P. et al. (2013) PLoS Genetics 9:e1003338.

4. Stützer, I. et al. (2013) J. Biol. Chem. 288:10536.

5. Gunnersen, J.M. et al. (2007) Neuron 56:621.

6. Gorlov, I.P. et al. (2007) Cancer Res. 67:8406.

7. Xu, C. et al. (2013) J. Affect. Disord. 145:95.

• Long Name:

  Seizure-related 6 Homolog-like

• Entrez Gene IDs:

  23544 (Human); 56747 (Mouse); 304554 (Rat)

• Alternate Names:

  Acig1; AIG1; Aig1L; BSRP-B; seizure related 6 homolog (mouse)-like; seizure related gene 6-like; SEZ6L