• Purity

  >95%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by the ability of the immobilized protein to support the adhesion of U‑87 MG human glioblastoma/astrocytoma cells. The ED    ₅₀ for this effect is 1-5 μg/mL.

• Source

  Chinese Hamster Ovary cell line, CHO-derived Cys19-Ser375, with a C-terminal 6-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  Cys19

• Predicted Molecular Mass

  40.9 kDa

• SDS-PAGE

  55-66 kDa, reducing conditions

Background: GPR111

GPR111, also known as PGR20, is an approximately 70 kDa member of the LN-7TM family of adhesion G protein-coupled receptors. Like other LN-7TM family proteins, it contains an extended N-terminal extracellular region followed by a series of seven transmembrane (TM) segments and a short C-terminal cytoplasmic tail (1, 2). The N-terminal domain contains a mucin-like stalk and a juxtamembrane region that resembles the GPS motif found in other LN-7TM proteins. The GPS motif is a component of the conserved GAIN domain which mediates the autoproteolysis and shedding of a wide range of proteins including LN-7TM proteins (3). The GPS-like motif in GPR111, however, is divergent from the consensus motif and does not appear to be cleavable (3, 4). Alternative splicing of human GPR111 generates a long isoform with a 92 aa substitution for the N-terminal 24 residues as well as a 19 aa substitution in the final TM segment and cytoplasmic tail. This recombinant protein product corresponds to the N-terminal extracellular domain of the shorter isoform (aa 19-375). Within this region, human GPR111 shares approximately 68% aa sequence identity with mouse and rat GPR111. GPR111 is expressed in squamous epithelia of the skin, esophagus, tongue epidermis, and stomach (4).

• References:

1. Fredriksson, R. et al. (2002) FEBS Lett. 531:407.

2. Bjarnadottir, T.K. et al. (2004) Genomics 84:23.

3. Arac, D. et al. (2012) EMBO J. 31:1364.

4. Promel, S. et al. (2012) Dev. Dyn. 241:1591.

• Long Name:

  G-Protein-Coupled Receptor 111

• Entrez Gene IDs:

  222611 (Human); 435529 (Mouse); 301269 (Rat)

• Alternate Names:

  G protein-coupled receptor 111; GPR111; PGR20