• Purity

  >95%, by SDS-PAGE with silver staining.

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its binding ability in a functional ELISA. When Recombinant Human IGFLR1 is coated at 0.1 μg/mL (100 μL/well), the concentration of recombinant human IGFL1 that produces 50% optimal binding response is 0.6-3 ng/mL.

• Source

  Chinese Hamster Ovary cell line, CHO-derived Ser23-Pro163 with a C-terminal 6-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  Ser23

• Predicted Molecular Mass

  16 kDa

• SDS-PAGE

  22-32 kDa, reducing conditions

Background: IGFLR1

Insulin Growth Factor-like Family Receptor 1 (IGFLR1) is a 355 amino acid (aa) type 1a transmembrane protein that was identified in a screen for binding partners of human IGFL1 (1). Mature human IGFLR1 consists of an extracellular domain (ECD) with two putative cysteine-rich domains (CRDs), a transmembrane region, and a cytoplasmic domain (1). Its structure has similarities to TNF receptor family members (1).  The ECD contains 12 cysteine residues that are conserved among humans, macaque, mice, and rats (1). Over the first 163 aa, human IGFLR1 shares 61% and 59% aa sequence identity with mouse and rat IGFLR1, respectively. In mice, IGFLR1 is expressed primarily on T cells and, similar to the human proteins, mouse IGFLR1 binds the mouse IGFL protein (1). Human IGFL1 expression is enhanced by TNF-alpha treatment and was shown to be up-regulated in human psoriatic skin samples, suggesting that IGFLR1 may have a role during skin inflammation (1).

• References:

1. Lobito, A.A. et al. (2011) J. Biol. Chem. 286:18969.

• Long Name:

  IGF-like Family Receptor 1

• Entrez Gene IDs:

  79713 (Human); 101883 (Mouse); 499126 (Rat)

• Alternate Names:

  FLJ22573; IGFLR1; TMEM149; transmembrane protein 149; U2 small nuclear RNA auxiliary factor 1-like 4; U2(RNU2) small nuclear RNA auxiliary factor 1-like 4; U2AF1L4