• Purity

  >95%, by SDS-PAGE with silver staining

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by the ability of the immobilized protein to support the adhesion of BCE C/D‑1b bovine corneal endothelial cells. The ED    ₅₀ for this effect is 0.04-0.2 μg/mL.    
     Optimal dilutions should be determined by each laboratory for each application.  

• Source

  Chinese Hamster Ovary cell line, CHO-derived Lys28-Ser699, with a C-terminal 6-­His tag

• Accession #

• N-terminal Sequence    
  Analysis

  Lys28

• Predicted Molecular Mass

  75 kDa

• SDS-PAGE

  90-105 kDa, reducing conditions

Background: Protocadherin-18

Protocadherin-18 (PCDH18) is an approximately 130 kDa glycoprotein belonging to the δ2 subgroup of non-clustered protocadherins (1-3). Like other δ2 protocadherins, mature Protocadherin-12 contains six cadherin domains in its extracellular domain (ECD), a transmembrane sequence, and a cytoplasmic domain (1, 2, 4). Within the ECD, human Protocadherin-18 shares 92% and 91% amino acid sequence identity with mouse and rat Protocadherin-18, respectively. Protocadherin-18 is expressed in the brain, heart, kidney, lung, and trachea (1, 5). Protocadherin-18 promotes cell-cell adhesion during embryogenesis in Zebrafish (6). Mouse Protocadherin-18 interacts with Disabled-1, a protein required for correct formation of cortical neuron layers (4, 5, 7, 8). Also in mice, Protocadherin-18 may additionally be an activation marker for CD8  ⁺ memorty T cells (9). In humans, deletion of Protocadherin-18 has been linked to intellectual disability (10).

• References:

1. Wolverton, T. and M. Lalande (2001) Genomics 76:66.

2. Vanhalst, K. et al. (2005) Cell. Mol. Life Sci. 62:1247.

3. Kim, S.Y. et al. (2011) Cell Adh. Migr. 5:97.

4. Redies, C. et al. (2005) Cell. Mol. Life Sci. 62:2840.

5. Homayouni, R. et al. (2001) Biochem. Biophys. Res. Commun. 289:539.

6. Aamar, E. and I.B. Dawid (2008) Dev. Biol. 318:335.

7. Howell, B.W. et al. (1999) Genes Dev. 13:643.

8. Howell, B.W. et al. (2000) Curr. Biol. 10:877.

9. Vazquez-Cintron, E.J. et al. (2012) PLoS One 7:e36101.

10. Kasnauskiene, J. et al. (2012) Eur. J. Med. Genet. 55:274.

• Entrez Gene IDs:

  54510 (Human); 73173 (Mouse); 295027 (Rat)

• Alternate Names:

  KIAA1562; PCDH68L1; protocadherin 18; Protocadherin18; Protocadherin-18