• Purity

  >95%, by SDS-PAGE with silver staining.

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its binding ability in a functional ELISA. When Recombinant Human TMIGD2/CD28H Fc Chimera is immobilized at 0.125 μg/mL, 100 μL/well, the concentration of Recombinant Human B7-H7  Fc Chimera (Catalog # ) that produces 50% of the optimal binding response is approximately 0.07-0.35 μg/mL.

• Source

  Human embryonic kidney cell, HEK293-derived

  Human TMIGD2 (Leu23-Gly150) Accession # Q96BF3	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  Leu23

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  41 kDa (monomer)

• SDS-PAGE

  57-63 kDa, reducing conditions

Background: TMIGD2/CD28H

CD28 homolog (CD28H), also called TMIG2 and IGPR-1, is a 55 kDa glycosylated transmembrane protein that shares approximately 10% amino acid (aa) sequence identity with CD28, CTLA-4, ICOS, and PD-1. CD28H is composed of a single extracellular immunoglobulin variable-like domain (IgV) (aa 23-109), a transmembrane domain (aa 151-171), and a long cytoplasmic domain (aa172-282). CD28H is constitutively expressed on naive T and NK cells. Similar to the interaction of B7 with CD28, the interaction of CD28H with B7-H7 activates the Akt-dependent signaling cascade and promotes the proliferation and activation of newly generated peripheral effector and memory T cells (1, 2). CD28H is additionally expressed in the skin and epithelium lining the lung, airway, mammary gland, and gastrointestinal tract (3). It regulates cellular morphology, focal adhesion contact formation, and cell migration (3, 4). CD28H also participates in angiogenesis   in vitro (3). CD28H interacts with multiple cytoskeletal proteins including Actin, Paxillin, SPIN90, CACNB2, and BPAG1. Interactions between the cytoplasmic proline-rich domain of CD28H and SPIN90 modulate the activity of CD28H in both angiogenesis and cell adhesion (3, 5).

• References:

1. Zhu, Y. et al. (2013) Nat. Commun. 4:2043.

2. Parry, R.V. et al. (2003) J.Immunol. 171:166.

3. Rahimi, N. et al. (2012) Mol. Biol. Cell. 23:1646.

4. Huang, C. et al. (2003) Nature 424:219.

5. Kaneko, T. et al. (2008) Front. Biosci. 13:4938.

• Long Name:

  Transmembrane And Immunoglobulin Domain Containing 2

• Entrez Gene IDs:

  126259 (Human)

• Alternate Names:

  CD28H; IGPR1; IGPR-1; MGC23244; TMIGD2; transmembrane and immunoglobulin domain containing 2; transmembrane and immunoglobulin domain-containing protein 2