• Purity

  >90%, by SDS-PAGE with silver staining.

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its binding ability in a functional ELISA. Measured by its ability to bind biotinylated Recombinant human/mouse Wnt-5a in a functional ELISA. The ED    ₅₀ for this effect is 1.5-9 µg/mL.

• Source

  Mouse myeloma cell line, NS0-derived

  Human ROR2 (Val28-Gly403) Accession # AAI30523	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• N-terminal Sequence    
  Analysis

  Val28

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  69 kDa

• SDS-PAGE

  80-92 kDa, reducing conditions

Background: ROR2

ROR2 (Receptor Tyrosine Kinase-like Orphan Receptor 2) is a member of the ROR family of receptor tyrosine kinases and is important for skeletal development, including bone and cartilage formation, as well as for the development of the central nervous system (1-5). Mature human ROR2 contains a 369 amino acid (aa) extracellular domain (ECD) and a 518 aa cytoplasmic tail containing an tyrosine kinase domain. The ROR2 ECD features an immunoglobulin domain, a cysteine-rich domain that resembles the Wnt-binding sites of Frizzled proteins, and a kringle domain. The ECD of human ROR2 shares 93% and 94% aa sequence identity with the ECD of mouse and rat ROR2, respectively. ROR2 binds the Wnt family ligand, Wnt-5a, to activate non-canonical Wnt signaling pathways (6-8). ROR2 is broadly expressed during embryonic development and can be found in cells of all three germ layers as well as in most organ tissues (9, 10). Activation of ROR2 signaling promotes cellular proliferation, differentiation, cell-polarization, and migration. ROR2 is important for osteogenic and chondrogenic differentiation of mesenchymal stem cells (11, 12). Mutations in ROR2 are associated with the skeletal disorders, brachydactyly type B and Robinow syndrome (6). ROR2 is also involved in neurite outgrowth and synapse development in the brain (4). A variety of tumors have been found to over-express ROR2. Depending upon the cellular context, ROR2 can act as a tumor suppressor or promoter (13). Expression of ROR2 within carcinomas is correlated with a poor prognosis of survival and increased rates of recurrence (14-16).

• References:

1. DeChiara, T.M. et al. (2000) Nat. Genet. 24:271.

2. Petrova, I.M. et al. (2014) Mol. Neurobiol. 49:303.

3. Takeuchi, S. et al. (2000) Genes Cells 5:71.

4. Paganoni, S. and A. Ferreira (2003) J. Neurosci. Res. 73:429.

5. Endo, M. et al. (2012) J. Cell. Sci. 125:2017.

6. Minami, Y. et al. (2010) Dev. Dyn. 239:1.

7. Oishi, I. et al. (2003) Genes Cells 8:645.

8. Wallkamm, V. et al. (2014) PLoS ONE 9:e109428.

9. Al-Shawi, R. et al. (2001) Dev. Genes. Evol. 211:161.

10. Matsuda, T. et al. (2001) Mech. Dev. 105:153.

11. Cai, S.X. et al. (2014) J. Cell. Physiol. 229:791.

12. Xin, H. et al. (2013) Int. J. Mol. Med. 31:583.

13. Ford, C.E. et al. (2013) Int. J. Cancer 133:779.

14. Rasmussen, N.R. et al. (2014) PLoS ONE 9:e116101.

15. Mei, H. et al. (2014) Biochem. Biophys. Res. Commun. 453:703.

16. Lu, B.J. et al. (2012) Mol. Med. Rep. 5:1033.

• Long Name:

  Receptor Tyrosine Kinase-like Orphan Receptor 2

• Entrez Gene IDs:

  4920 (Human)

• Alternate Names:

  BDB; BDB1; EC 2.7.10.1; MGC163394; neurotrophic tyrosine kinase receptor-related 2; Neurotrophic tyrosine kinase, receptor-related 2; NTRKR2; NTRKR2tyrosine-protein kinase transmembrane receptor ROR2; receptor tyrosine kinase-like orphan receptor 2; ROR2