• Species Reactivity

  Human

• Specificity

  Detects human UVRAG in direct ELISAs.

• Source

  Monoclonal Mouse IgG1 Clone # 953035

• Immunogen

  Human UVRAG synthetic peptide corresponding to sequences between amino acids 260-360    
  Accession # Q9P2Y5

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 405

Applications

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  Recommended    
  Concentration

  Sample

• Intracellular Staining by Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  Raji human Burkitt's lymphoma cell line and Daudi human Burkitt's lymphoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # ) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # )

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Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: UVRAG

UV radiation resistance-associated gene protein, or UVRAG, is a multi-function protein of 699 amino acids involved in the regulation of cellular pathways implicated in membrane trafficking. UVRAG activates the Beclin-1-PI3KC3 complex, a critical element in the autophagy signaling pathway. Binding studies show that UVRAG and Beclin-1 directly interact via their coiled-coil domains and that Beclin-1 functions as a platform for the formation of the Bcl-2-UVRAG-PI3KC3 complex. Chromosomal aberrations involving the UVRAG gene are associated with left-right axis malformation, and mutations in the gene have been associated with colon cancer.

• Long Name:

  UV Radiation Resistance Associated

• Entrez Gene IDs:

  7405 (Human); 78610 (Mouse); 308846 (Rat)

• Alternate Names:

  beclin 1 binding protein; DHTX; disrupted in heterotaxy; p63; UV radiation resistance associated gene; UV radiation resistance-associated gene protein; UVRAG; VPS38