详细说明
Species Reactivity
Human
Specificity
Detects chimeric proteins containing a human IgG Fc region in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG 1 Clone # 97924
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IgG 1 Fc region
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin
Applications
Recommended
ConcentrationSample
Flow Cytometry
10 µL/10 6 cells
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Flow Cytometry | Detection of IgG1 Fc in Human B7-H1/PD-L1 Fc Chimera Bound to HEK293 Human Cell Line Transfected with Human PD-1 and eGFP by Flow Cytometry. Recombinant Human B7‑H1/PD‑L1 Fc Chimera (Catalog # ) bound to HEK293 human embryonic kidney cell line transfected with human PD-1 and eGFP was stained with either (A) Mouse Anti-Human IgG Fc APC‑conjugated Monoclonal Antibody (Catalog # FAB110A) or (B) Mouse IgG1 Allophycocyanin Isotype Control (Catalog # ). Panel C shows HEK293 cell line transfected with human PD-1, untreated with B7-H1 Fc Chimera, and stained with Mouse Anti-Human IgG Fc APC‑conjugated Monoclonal Antibody (Catalog # FAB110A). View our protocol for . |
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Long Name:
Immunoglobulin G
Alternate Names:
IgG; immunoglobulin heavy constant gamma 1 (G1m marker)