详细说明
- Species ReactivityHuman
- SpecificityDetects human LAP (TGF-beta 1) in direct ELISAs. In direct ELISAs, this antibody does not cross-react with recombinant human (rh) TGF‑ beta 1, rhTGF‑ beta 2, rhTGF‑ beta 1.2, rhTGF‑ beta 3, or rhTGF‑ alpha.
- SourceMonoclonal Mouse IgG 1 Clone # 27232
- PurificationProtein A or G purified from hybridoma culture supernatant
- ImmunogenS. frugiperda insect ovarian cell line Sf 21-derived recombinant human LAP (TGF-beta 1)
Leu30-Arg278 (Cys33Ser)
Accession # P01137 - FormulationSupplied in a saline solution containing BSA and Sodium Azide.
- LabelAlexa Fluor 488
- Recommended
ConcentrationSample
- Flow Cytometry5 µL/10 6 cellsSee below
- Immunocytochemistry5-15 µg/mLSee below
Flow Cytometry | Detection of LAP (TGF-beta 1) in Human Platelets by Flow Cytometry. Human platelets were stained with Mouse Anti-Human LAP (TGF-beta 1) Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB2463G, filled histogram) or isotype control antibody (Catalog # , open histogram). View our protocol for . |
Immunocytochemistry | LAP (TGF-beta 1) in Human PBMCs. LAP (TGF-beta 1) was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with CD3/CD28/TGF-beta/IL-2 using Mouse Anti-Human LAP (TGF-beta 1) Alexa Fluor® 488‑conjugated Monoclonal Antibody (green; Catalog # FAB2463G) at 5 µg/mL for 3 hours at room temperature. Cells were counterstained with DAPI (blue). View our protocol for . |
- ShippingThe product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
- Stability & StorageProtect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
TGF-beta 1 is one member of a six gene family that has been described in mammals, birds, fish and frog. The name TGF-beta is applied to a 24-28 kDa disulfide-linked dimer that is generated through the proteolytic processing of a larger precursor molecule. For TGF-beta 1, a 50-55 kDa, 391 amino acid (aa) proprecursor is first, covalently linked to a second proprecursor (creating a disulfide-linked homodimer), and second, internally cleaved to generate two covalently-linked homodimers that remain non-covalently associated. The smallest homodimer representing aa 279-390 of the proform is TGF-beta 1; the largest homodimer representing aa 30-278 of the proform is termed LAP (Latency-associated Peptide). The LAP homodimer wraps itself around the smaller TGF-beta 1 homodimer, thus blocking an interaction of mature TGF-beta with its receptors. Almost all cells secrete the inactive TGF-beta :LAP complex, and most do so with LAP covalently bound to a very large 120-160 kDa LTBP (Latent TGF-beta Binding Protein), platelets being a notable exception. LTBP associates with multiple matrix components and this serves to store TGF-beta extracellularly in a non-active form. When TGF-beta signaling is needed, the LTBP:matrix association is disrupted, and the TGF-beta :LAP complex is exposed to multiple LAP binding partners such as TSP-1 and various Integrins. Interactions with these factors cause LAP to unwrap and dissociate from TGF-beta, resulting in TGF-beta "activation" and receptor binding. Ther are three human TGF-beta 1 LAPs and TGF-beta 1 LAP shares 36% and 33% aa sequence indentity with TGF-beta 2 LAP and TGF-beta 3 LAP, respectively. Human to mouse, TGF-beta 1 LAP shares 86% aa sequence identity.
- Long Name:Latency-associated Peptide
- Entrez Gene IDs:7040 (Human)
- Alternate Names:LAP (TGFbeta 1); LAP (TGF-beta 1)