详细说明
- Assay TypeSolid Phase Sandwich ELISA
- Format96-well strip plate
- Assay Length4 hours 40 mins (after plate preparation)
- Sample Type & Volume RequiredCell lysates (100 µL)
- Range312.00 - 20,000 pg/mL
- Sufficient MaterialsKits available for two, five, or fifteen 96-well plates*
- SpecificityPlease see the
This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Available in 2, 5, and 15- (96-well) plate pack sizes
- Economical alternative to Western blot
- Capture Antibody
- Conjugated Detection Antibody
- Calibrated Immunoassay Standard or Control
- Streptavidin-HRP
PBS: (Catalog # ), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na 2HPO 4, 1.5 mM KH 2O 4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # ), or equivalent
Lysis Buffer*
IC Diluent*
Blocking Buffer*
Substrate Solution: 1:1 mixture of Color Reagent A (H 2O 2) and Color Reagent B (Tetramethylbenzidine) (Catalog # )
Stop Solution: 2 N H 2SO 4 (Catalog # )
Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # ), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # ), or equivalent
*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product
| Figure 1: Specificity of Human Total MDM2 Duoset IC ELISA is shown by Western blot. Lysates prepared from LLnV treated U2OS human osteosarcoma cells were incubated in wells coated with Human Total MDM2 Capture Antibody. Unbound material was removed by washing and bound material was solubilized in SDS gel sample buffer. The same lysate and captured proteins were electrophoresed, transferred to a PVDF membrane, and immunoblotted with Human Total MDM2 Detection Antibody. The captured protein corresponds to MDM2 detected in the total cell lysates. |
| Figure 2: Amounts of MDM2, as quantified by the Human Total MDM2 Duoset IC ELISA, are consistent with the relative levels of MDM2 determined by qualitative Western blot analysis. Lysates prepared from U2OS human osteosarcoma +/- LLnV cells were quantified with this DuoSet® IC. The same lysates were also immunoblotted (inset) with a monoclonal detection antibody. The DuoSet® IC results correlate well with the relative amounts of human MDM2 detected by Western Blot. |
- StorageStore the unopened product at 2 - 8 °C. Do not use past expiration date.
- Entrez Gene IDs:4193 (Human); 17246 (Mouse);
- Long Name:Double Minute 2 Protein
- Aliases:ACTFS; HDM2; HDMX; MDM2 oncogene, E3 ubiquitin protein ligase; MDM2
400-6226-992