详细说明
- Assay TypeSolid Phase Sandwich ELISA
- Format96-well strip plate
- Sample Type & Volume RequiredCell lysates (100 µL)
- Range156.00 - 10,000 pg/mL
- Sufficient MaterialsKits available for two, five, or fifteen 96-well plates*
- SpecificityPlease see the
This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure phosphorylated
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Available in 2, 5, and 15- (96-well) plate pack sizes
- Economical alternative to Western blot
- Capture Antibody
- Conjugated Detection Antibody
- Calibrated Immunoassay Standard or Control
- Streptavidin-HRP
PBS: (Catalog # ), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na 2HPO 4, 1.5 mM KH 2O 4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # ), or equivalent
Lysis Buffer*
IC Diluent*
Blocking Buffer*
Substrate Solution: 1:1 mixture of Color Reagent A (H 2O 2) and Color Reagent B (Tetramethylbenzidine) (Catalog # )
Stop Solution: 2 N H 2SO 4 (Catalog # )
Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # ), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # ), or equivalent
*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product
- StorageStore the unopened product at 2 - 8 °C. Do not use past expiration date.
- Long Name:C-Jun N-terminal Kinase
- Aliases:c-Jun N-terminal kinase 1; EC 2.7.11; EC 2.7.11.24; JNK; JNK1 alpha protein kinase; JNK1 beta protein kinase; JNK1JNK1A2; JNK-46; JUN N-terminal kinase; MAP kinase 8; MAPK 8; mitogen-activated protein kinase 8 isoform JNK1 alpha1; mitogen-activated protein kinase 8 isoform JNK1 beta2; mitogen-activated protein kinase 8; PRKM8JNK; protein kinase JNK1; SAPK1JNK21B1/2; Stress-activated protein kinase 1; Stress-activated protein kinase JNK1