Human Total IGF-I R DuoSet IC ELISA, 2 Plate 1 KT

所属行业:生物试剂     有0人感兴趣

Human Total IGF-I R DuoSet IC ELISA, 2 Plate 1 KT信息二维码

微信扫码

 
基础投资电议
推荐指数
  • 区域全国
  • 留言暂无
  • 浏览0
  • 更新2024-07-25 20:28
  • 到期长期有效






更多>我还有产品
3-Amino-5-methoxycarbonylphenylboronic acid, pinacol ester  1 g 2,3-Dichloro-6-(trifluoromethyl)benzyl bromide  1 g 1-(4-Fluorophenyl)-5-methoxycarbonyl-2(1H)-pyridinone  1 g N,N-Diethyl-cyclohexane-1,4-diamine  1 g N-Methyl-DL-leucine hydrochloride  5 g N-(2-Chloroethyl) 3-boronobenzamide  5 g

产品介绍

    基本参数

    详细说明

    • Assay Type
      Solid Phase Sandwich ELISA
    • Format
      96-well strip plate
    • Assay Length
      4 hours 40 mins (after plate preparation)
    • Sample Type & Volume Required
      Cell lysates (100 µL)
    • Range
      156.00 - 10,000 pg/mL
    • Sufficient Materials
      Kits available for two, five, or fifteen 96-well plates*
    • Specificity
      Please see the
    * Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

    This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure in cell lysates. An immobilized capture antibody specific for binds both phosphorylated and unphosphorylated . After washing away unbound material, a biotinylated detection antibody is used to detect both phosphorylated and unphosphorylated protein, utilizing a standard Streptavidin-HRP format.


    Product Features
    • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
    • Development protocols are provided to guide further assay optimization
    • Assay can be customized to your specific needs
    • Available in 2, 5, and 15- (96-well) plate pack sizes
    • Economical alternative to Western blot
    Kit Content
    • Capture Antibody
    • Conjugated Detection Antibody
    • Calibrated Immunoassay Standard or Control
    • Streptavidin-HRP
    Other Reagents Required

    PBS: (Catalog # ), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na 2HPO 4, 1.5 mM KH 2O 4, pH 7.2 - 7.4, 0.2 µm filtered

    Wash Buffer: (Catalog # ), or equivalent

    Lysis Buffer*

    IC Diluent*

    Blocking Buffer*


    Substrate Solution: 1:1 mixture of Color Reagent A (H 2O 2) and Color Reagent B (Tetramethylbenzidine) (Catalog # )

    Stop Solution: 2 N H 2SO 4 (Catalog # )

    Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # ), or equivalent

    Plate Sealers: ELISA Plate Sealers (Catalog # ), or equivalent

    *For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

    Preparation and Storage
    • Storage
      Store the unopened product at 2 - 8 °C. Do not use past expiration date.
    Background: IGF-I R

    IGF-I receptor is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha and two beta subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The alpha subunit of IGF-I receptor is extracellular while the beta subunit has an extracellular domain, a transmembrane domain and a cytoplasmic tyrosine kinase domain. The IGF-I receptor is highly expressed in all cell types and tissues.

    IGF-II R is a type I transmembrane glycoprotein that contains a 2,264 amino acid (aa) extracellular region, a 23 aa transmembrane segment segment and a 124 aa cytoplasmic tail. IGF-II R regulates many diverse biological functions that range from intracellular trafficking to the internalization of extracellular factors and modulation of cellular responses. It delivers newly synthesized M6P-tagged lysosomal enzymes from the trans-golgi network to endosomes, and facilitates the clearance of extracellular lysosomal and matrix degrading enzymes by internalization into clathrin-coated vesicles and delivery into endosomes. With respect to IGF-II biology, It would appear that IGF-II R is principally a regulator of local IGF-II levels, targeting IGF-II for destruction in lysosomes.

    The heterotetrameric receptors for insulin (INS R) and IGF-I (IGF-I R) are receptor tyrosine kinases that consist of two ligandbinding alpha subunits and two beta subunits. Ligand binding induces autophosphorylation on multiple tyrosine residues of beta subunits. Phosphorylation of Tyr1162 and 1163 on INS R and Tyr1135 and 1136 on IGF-I R stimulates intrinsic kinase activity.

    • Entrez Gene IDs:
      3480 (Human);
    • Long Name:
      Insulin-like Growth Factor I Receptor
    • Aliases:
      CD221 antigen; CD221; EC 2.7.10; EC 2.7.10.1; IGF1R; IGF-I R; IGF-I receptor; IGFIR; IGF-IR; IGFR; insulin-like growth factor 1 receptor; Insulin-like growth factor I receptor; JTK13; MGC142170; MGC142172; MGC18216; soluble IGF1R variant 1; soluble IGF1R variant 2
    举报收藏 0评论 0
 
热门项目排行榜快速发布信息
14-(2-碘代乙基)辛基苯 9
2高温波导测试
3格列美脲 98%+ RG  5g
44-溴-3,5-二氯三氟甲苯
5二次谐波
6远红外云试剂
生物试剂排行榜快速发布信息
1Recombinant Human LILR
2Recombinant Human CD47
3Recombinant Human FGF
4203mm具砂板闪式层析柱,
5pUCm-T 载体 5UG
65ml Protein A40 Plus亲
最新加入项目快速发布信息
1质粒大量抽提试剂盒(通
2实验试剂
3产品标题产品标题产品标
4实验试剂
53-Amino-5-methoxycarbo
6N,N-Diethyl-cyclohexan

猜你喜欢产品快速发布信息

相关栏目

还没找到您需要的生物试剂产品?立即发布您的求购意向,让生物试剂公司主动与您联系!

立即发布项目意向

免责声明

本网页所展示的有关【Human Total IGF-I R DuoSet IC ELISA, 2 Plate 1 KT_生物试剂_云试剂自营四店】的信息/图片/参数等由云试剂的会员【云试剂自营四店】提供,由云试剂会员【云试剂自营四店】自行对信息/图片/参数等的真实性、准确性和合法性负责,本平台(本网站)仅提供展示服务,请谨慎交易,因交易而产生的法律关系及法律纠纷由您自行协商解决,本平台(本网站)对此不承担任何责任。您在本网页可以浏览【Human Total IGF-I R DuoSet IC ELISA, 2 Plate 1 KT_生物试剂_云试剂自营四店】有关的信息/图片/价格等及提供【Human Total IGF-I R DuoSet IC ELISA, 2 Plate 1 KT_生物试剂_云试剂自营四店】的商家公司简介、联系方式等信息。

联系方式

在您的合法权益受到侵害时,欢迎您向www@yunshiji.cn邮箱发送邮件,或者进入《网站意见反馈》了解投诉处理流程,我们将竭诚为您服务,感谢您对云试剂的关注与支持!

按排行字母分类找加盟项目:

A B C D E F G H I J K L M N O P Q R S T U V W X Y Z